Enzymes Recombinant Proteins

Human KLK7 Recombinant Protein (RPPB1868)

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SKU:
RPPB1868
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49862
Research Area:
Enzymes

Description

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Product Name:Human KLK7 Recombinant Protein
Product Code:RPPB1868
Size:5µg
Species:Human
Target:KLK7
Synonyms:KallikreinRelated Peptidase 7, Kallikrein 7 (Chymotryptic, Stratum Corneum), StratumCorneum Chymotryptic Enzyme, Serine Protease 6, PRSS6, SCCE, HK7,Kallikrein-Related Peptidase 7, Signal Protein, EC 3.4.21.117, EC 3.4.21,HSCCE.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:KLK7 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPMNEYTVH LGSDTLGDRR AQRIKASKSF RHPGYSTQTH VNDLMLVKLNSQARLSSMVK KVRLPSRCEP PGTTCTVSGW GTTTSPDVTF PSDLMCVDVK LISPQDCTKV YKDLLENSMLCAGIPDSKKN ACNGDSGGPL VCRGTLQGLV SWGTFPCGQP NDPGVYTQVC KFTKWINDTM KKHRHHHHHH

KLK7 catalyzes the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. KLK7 cleaves insulin B chain at ''6-Leu- -Cys-7'', ''16-Tyr- -Leu-17'', ''25-Phe- -Tyr-26'' and ''26-Tyr--Thr-27''. KLK7 is involved in the activation of precursors to inflammatory cytokines.

KLK7 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 190 amino acids (1-181 a.a.) andhaving a molecular mass of 20.9kDa (Migrates at 28-40kDa on SDS-PAGE underreducing conditions).�KLK7 is expressed with a 6 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:KLK7: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. SCCE cleaves insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; EC 3.4.21.35; Protease; Secreted

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; extracellular space

Molecular Function:metalloendopeptidase activity; peptidase activity; serine-type peptidase activity

Biological Process: epidermis development; extracellular matrix disassembly; positive regulation of antibacterial peptide production

NCBI Summary:This gene encodes a member of the kallikrein subfamily of serine proteases. These enzymes have diverse physiological functions and many kallikrein genes are biomarkers for cancer. The encoded protein has chymotrypsin-like activity and plays a role in the proteolysis of intercellular cohesive structures that precedes desquamation, the shedding of the outermost layer of the epidermis. The encoded protein may play a role in cancer invasion and metastasis, and increased expression of this gene is associated with unfavorable prognosis and progression of several types of cancer. Polymorphisms in this gene may play a role in the development of atopic dermatitis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, which is one of fifteen kallikrein subfamily members located in a gene cluster on chromosome 19. [provided by RefSeq, May 2011]
UniProt Code:P49862
NCBI GenInfo Identifier:1710878
NCBI Gene ID:5650
NCBI Accession:P49862.1
UniProt Secondary Accession:P49862,Q8N5N9, Q8NFV7, A8K0U5,
UniProt Related Accession:P49862
Molecular Weight:19,887 Da
NCBI Full Name:Kallikrein-7
NCBI Synonym Full Names:kallikrein related peptidase 7
NCBI Official Symbol:KLK7��
NCBI Official Synonym Symbols:hK7; SCCE; PRSS6��
NCBI Protein Information:kallikrein-7
UniProt Protein Name:Kallikrein-7
UniProt Synonym Protein Names:Serine protease 6; Stratum corneum chymotryptic enzyme; hSCCE
Protein Family:Kallikrein
UniProt Gene Name:KLK7��
UniProt Entry Name:KLK7_HUMAN
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