Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to JAM-A-Fc coated plates (2.5µg/mL; 100 ?L/well)in the presence of 20 ng/mL PMA; approximately 30-40% will adhere after 30 minutes at 37?.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetHuman JAM-A/F11R Recombinant Protein Junctional Adhesion Molecule A (JAM-A) is a single-pass type I membrane protein that belongs to the immunoglobulin superfamily. JAM-
system_update_altDatasheetHuman JAM-A/F11R Recombinant Protein Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembr
system_update_altDatasheetRat JAM-A/F11R Recombinant Protein Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembran
system_update_altDatasheetRat JAM-A/F11R Recombinant Protein Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembran