Growth Factors & Cytokines Recombinant Proteins
Human Interleukin-7/IL-7 Recombinant Protein (RPES2582)
- SKU:
- RPES2582
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Growth Factors & Cytokines
Description
Human Interleukin-7/IL-7 Recombinant Protein
Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
| Product Name: | Human Interleukin-7/IL-7 Recombinant Protein (RPES2582) |
| Product Code: | RPES2582 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | Interleukin-7, IL-7, IL7 |
| Accession: | P13232 |
| Sequence: | Asp26-His177 |
| Fusion tag: | C-6His |
| Activity: | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 50-300 pg/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Asp26-His177 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 18.4 kDa |
| AP Mol Mass: | 25 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl; pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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