Human Insulin Receptor Pathway Phosphorylation Array (9 targets) (SARB0053)

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SKU:
SARB0053
Product Type:
Phosphorylation Array
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku: SARB0053
Size: 2, 4, or 8
Species Detected: Human
Number of Targets Detected: 9
Gene Symbols: EIF4E, FOXO3, IGF1R, INPP5D, INS, IRS1, PTPN11, SHC1, STK11
Compatible Sample Types: Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Chemiluminescence
Pathway: Akt Signaling / IGF Signaling / mTOR Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative: Semi-Quantitative
Solid Support: Membrane
Suggested Application(s): Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
eIF-4E (P-Ser209)
  • Gene Symbol: EIF4E EIF4EL1 EIF4F
  • Gene ID: 1977
  • Accession #: P06730
 FOXO-3 (P-Ser413)
  • Gene Symbol: FOXO3
  • Gene ID: 2309
  • Accession #: O43524
 IGF-1 R (P-Tyr1165)
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
 Insulin (P-Tyr1189)
  • Gene Symbol:INS
  • Gene ID: 3630
  • Accession #: P01308
 IRS-1 (P-Ser318)
  • Gene Symbol: IRS1
  • Gene ID: 3667
  • Accession #: P35568
LKB1 (P-Ser428)
  • Gene Symbol: STK11 LKB1 PJS
  • Gene ID: 6794
  • Accession #: Q15831
 SHC (P-Tyr427)
  • Gene Symbol: SHC1 SHC SHCA
  • Gene ID: 6464
  • Accession #: P29353
 SHIP1 (P-Tyr1020)
  • Gene Symbol: INPP5D SHIP SHIP1
  • Gene ID: 3635
  • Accession #: Q92835
 SHP-2 (P-Ser542)
  • Gene Symbol: PTPN11 PTP2C SHPTP2
  • Gene ID: 5781
  • Accession #: Q06124
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Insulin Receptor Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
    1. Block membranes
    2. Incubate with Sample
    3. Incubate with Detection Antibody Cocktail
    4. Incubate with HRP-Conjugated anti-IgG
    5. Incubate with Detection Buffers
    6. Image with chemiluminescent imaging system
    7. Perform densitometry and analysis
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