Human Immune Checkpoint Array (12 targets) (SARB0049)

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SKU:
SARB0049
Product Type:
Protein Array
Analytes:
4-1BB (TNFRSF9/CD137)
Analytes:
B7-H2 (ICOS Ligand)
Analytes:
B7-H3 (CD276)
Analytes:
B7-H4
Analytes:
CD200
Analytes:
HVEM (TNFRSF14/CD270)
Analytes:
CD27 Ligand (TNFSF7)
Analytes:
CD80 (B7-1)
Analytes:
CD86 (B7-2)
Analytes:
OX40 Ligand (TNFSF4)
Analytes:
B7-H1 (CD274/PD-L1)
Analytes:
PD-L2 (B7-DC)
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0049
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:12
Gene Symbols:CD200, CD274, CD276, CD70, CD80, CD86, ICOSLG, PDCD1LG2, TNFRSF14, TNFRSF9|TNFSF4, VTCN1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4-1BB (TNFRSF9/CD137)
  • Gene Symbol: TNFRSF9 CD137 ILA
  • Gene ID: 3604
  • Accession #: Q07011
B7-H2 (ICOS Ligand)
  • Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653
  • Gene ID: 102723996; 23308
  • Accession #: O75144
B7-H3 (CD276)
  • Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352
  • Gene ID: 80381
  • Accession #: Q5ZPR3
B7-H4
  • Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291
  • Gene ID: 79679
  • Accession #: Q7Z7D3
CD200
  • Gene Symbol: CD200 MOX1 MOX2 My033
  • Gene ID: 4345
  • Accession #: P41217
HVEM (TNFRSF14/CD270)
  • Gene Symbol: TNFRSF14 HVEA HVEM UNQ329/PRO509
  • Gene ID: 8764
  • Accession #: Q92956
CD27 Ligand (TNFSF7)
  • Gene Symbol: CD70 CD27L CD27LG TNFSF7
  • Gene ID: 970
  • Accession #: P32970
CD80 (B7-1)
  • Gene Symbol: CD80 CD28LG CD28LG1 LAB7
  • Gene ID: 941
  • Accession #: P33681
CD86 (B7-2)
  • Gene Symbol: CD86 CD28LG2
  • Gene ID: 942
  • Accession #: P42081
OX40 Ligand (TNFSF4)
  • Gene Symbol: TNFSF4 TXGP1
  • Gene ID: 7292
  • Accession #: P23510
B7-H1 (CD274/PD-L1)
  • Gene Symbol: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1
  • Gene ID: 29126
  • Accession #: Q9NZQ7
PD-L2 (B7-DC)
  • Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2
  • Gene ID: 80380
  • Accession #: Q9BQ51
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Immune Checkpoint C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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