Growth Factors & Cytokines Recombinant Proteins
Human IL4RA/CD124 Recombinant Protein (RPES2442)
- SKU:
- RPES2442
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Growth Factors & Cytokines
Description
Human IL4RA/CD124 Recombinant Protein
Interleukin 4 Receptor alpha (IL4-Ra) is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. Mature human IL4-Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain; a 24 aa transmembrane segment; and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif. IL4-Ra plays an important role in Th2-biased immune responses; alternative macrophage activation; mucosal immunity; allergic inflammation; tumor progression; and atherogenesis. Soluble forms of IL4-Ra; generated by alternate splicing or proteolysis; retain ligand binding properties and inhibit IL-4 bioactivity. IL4-Ra is a component of two distinct receptor complexes and shows species selectivity between human and mouse. It can associate with the common gamma chain (?c) to form the IL-4 responsive type I receptor in which ?c increases the affinity for IL-4 and enables signaling. It can alternatively associate with IL13-Ra1 to form the type II receptor which is responsive to both IL-4 and IL-13. The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize ?c.
| Product Name: | Human IL4RA/CD124 Recombinant Protein (RPES2442) |
| Product Code: | RPES2442 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, IL-4-binding protein, IL4-BP, IL4R, IL4RA |
| Accession: | P24394 |
| Sequence: | Met26-His232 |
| Fusion tag: | C-6His |
| Activity: | Measured by its ability to inhibit IL-4-dependent proliferation of TF?1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Met26-His232 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 24.4 kDa |
| AP Mol Mass: | 40-60 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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