Cytokines Recombinant Proteins

Human IL36A 158 a.a. Recombinant Protein (RPPB0677)

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SKU:
RPPB0677
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UHA7
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human IL36A 158 a.a. Recombinant Protein
Product Code:RPPB0677
Size:10µg
Species:Human
Target:IL36A 158 a.a.
Synonyms:Interleukin 36 alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2?m filtered concentrated solution in 2xPBS, pH 7.4.
Solubility:It is recommended to reconstitute the lyophilized IL36A 158 a.a. Human in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IL36A 158 a.a. Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36A 158 a.a. Human should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Amino Acid Sequence:MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF
Biological Activity:Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36? at 1 ?g/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5?g/ml corresponding to a specific activity of 200,000-6,666,667IU/mg.

Human IL-36a belongs to the IL-1 family which includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7) and IL-38 (IL-1F10). The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. IL-36a is an 18-22kDa, 158aa intracellular and secreted protein which holds no signal sequence, no prosegment and no potential from N-linked glycosylation sites. IL-36a is released as a reaction to LPS and the cell ATP-induced activation of the P2X7 receptor. Human IL-36a (aa 6-158) shares 57-68% aa sequence homology with mouse, rabbit, equine and bovine IL-36a and 27-57% aa sequence homology with other new IL-1 family members. IL-36a is mostly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine.

IL36A 158 a.a. Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.7kDa.The IL36A 158 a.a. Human is purified by proprietary chromatographic techniques.

UniProt Protein Function:IL1F6: Belongs to the IL-1 family.
UniProt Protein Details:

Protein type:Cytokine

Chromosomal Location of Human Ortholog: 2q12-q14.1

Cellular Component: extracellular region; extracellular space

Molecular Function:cytokine activity; interleukin-1 receptor binding

Biological Process: cytokine and chemokine mediated signaling pathway

NCBI Summary:The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. [provided by RefSeq, Nov 2015]
UniProt Code:Q9UHA7
NCBI GenInfo Identifier:25008601
NCBI Gene ID:27179
NCBI Accession:Q9UHA7.1
UniProt Secondary Accession:Q9UHA7,Q53SR7, Q5BLR4, Q7RTZ8, B2RAD9,
UniProt Related Accession:Q9UHA7
Molecular Weight:17,684 Da
NCBI Full Name:Interleukin-36 alpha
NCBI Synonym Full Names:interleukin 36, alpha
NCBI Official Symbol:IL36A��
NCBI Official Synonym Symbols:FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)��
NCBI Protein Information:interleukin-36 alpha
UniProt Protein Name:Interleukin-36 alpha
UniProt Synonym Protein Names:FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6
Protein Family:Interleukin
UniProt Gene Name:IL36A��
UniProt Entry Name:IL36A_HUMAN
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