Cytokines Recombinant Proteins

Human IL1R2 Recombinant Protein (RPPB0478)

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SKU:
RPPB0478
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P27930
Research Area:
Cytokines

Description

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Product Name:Human IL1R2 Recombinant Protein
Product Code:RPPB0478
Size:5µg
Species:Human
Target:IL1R2
Synonyms:IL1R2, CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2, IL1R2c, IL1RB, �IL-1 type II receptor, �Interleukin-1 receptor beta, �CD121 antigen-like family member B.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:IL1R2 protein solution (0.5mg/ml) contains PBS (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:FTLQPAAHTG AARSCRFRGR HYKREFRLEG EPVALRCPQV PYWLWASVSP RINLTWHKND SARTVPGEEE TRMWAQDGAL WLLPALQEDS GTYVCTTRNA SYCDKMSIEL RVFENTDAFL PFISYPQILT LSTSGVLVCP DLSEFTRDKT DVKIQWYKDS LLLDKDNEKF LSVRGTTHLLVHDVALEDAG YYRCVLTFAH EGQQYNITRS IELRIKKKKE ETIPVIISPL KTISASLGSR LTIPCKVFLG TGTPLTTMLW WTANDTHIES AYPGGRVTEG PRQEYSENNE NYIEVPLIFD PVTREDLHMD FKCVVHNTLS FQTLRTTVKE LEHHHHHH
Biological Activity:Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is ? to 400 ng/ml with IL-1beta.

IL1R2 is a cytokine receptor which is a part of the interleukin-1 receptor family. IL1R2 is expressed mainly by T cells, endothelial cells and fibroblasts. IL1R2 binds IL1A, IL1B and IL1RN preventing them from binding to their regular receptors and thus inhibiting the transduction of their signaling. Also, Interleukin-4 antagonizes the activity of interleukin-1 by inducing the expression and release of this protein.

IL1R2 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 338 amino acids (14-343a.a.) and having a molecular mass of 38.8kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).IL1R2 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:IL-1RB: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: plasma membrane

Molecular Function:interleukin-1 receptor activity; protein binding

Biological Process: immune response

NCBI Summary:The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]
UniProt Code:P27930
NCBI GenInfo Identifier:124310
NCBI Gene ID:7850
NCBI Accession:P27930.1
UniProt Secondary Accession:P27930,Q6LCE6, Q9UE68, D3DVJ5,
UniProt Related Accession:P27930
Molecular Weight:33,622 Da
NCBI Full Name:Interleukin-1 receptor type 2
NCBI Synonym Full Names:interleukin 1 receptor type 2
NCBI Official Symbol:IL1R2��
NCBI Official Synonym Symbols:IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2��
NCBI Protein Information:interleukin-1 receptor type 2
UniProt Protein Name:Interleukin-1 receptor type 2
UniProt Synonym Protein Names:CD121 antigen-like family member B; CDw121b; IL-1 type II receptor; Interleukin-1 receptor beta; IL-1R-beta
Protein Family:Interleukin-1 receptor
UniProt Gene Name:IL1R2��
UniProt Entry Name:IL1R2_HUMAN
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