Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
Product Name:
Human IL1R1/CD121a Recombinant Protein (RPES1697)
Product Code:
RPES1697
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD121A,D2S1473,IL-1 RI,IL-1R-alpha,IL-1R1,IL1R,IL1RA,P80,Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, IL-1R-alpha
Accession:
NP_000868.1
Sequence:
Met 1-Thr 332
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized human IL1-beta at 20 µg/ml (100 ?l/well) can bind human IL1R1 Fc chimera with a linear ranger of 6.4-160 ng/ml.2. Measured by its binding ability in a functional ELISA.3. Immobilized human IL1B-his at 10 µg/mL (100 ?l/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.198 µg/mL.4. Immobilized human IL1B at 10 µg/mL (100 ?l/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.48 µg/mL.5. Measured by its ability to bind canine IL1B in a functional ELISA.6. Measured by its ability to bind mouse IL1B in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of human IL1R1 (NP_000868.1) (Met 1-Thr 332) was fused with human IgG1 Fc region at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
63 kDa
AP Mol Mass:
80-90 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetHuman IL1R1/CD121a Recombinant Protein Interleukin 1 receptor, type I (IL-1R1) is an interleukin receptor that belongs to the interleukin-1 receptor family. IL-1R1 is an
system_update_altDatasheetHuman IL1R1/CD121a Recombinant Protein Interleukin 1 receptor, type I (IL-1R1) is an interleukin receptor that belongs to the interleukin-1 receptor family. IL-1R1 is an
system_update_altDatasheetHuman IL1R1/CD121a Recombinant Protein Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1
system_update_altDatasheetRat IL1R1/CD121a Recombinant Protein Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/C
