Cytokines Recombinant Proteins

Human IL15RA Recombinant Protein (RPPB0590)

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RPPB0590

Description

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Product Name:Human IL15RA Recombinant Protein
Product Code:RPPB0590
Size:10µg
Species:Human
Target:IL15RA
Synonyms:Interleukin 15 Receptor Subunit Alpha, Interleukin 15 Receptor, Alpha, Interleukin 15 Receptor Alpha Isoform EM2, Interleukin 15 Receptor Alpha Isoform IC2, Interleukin 15 Receptor Alpha Isoform IC3, Interleukin 15 Receptor Alpha Isoform IC4, Interleukin 15 Receptor Alpha Isoform IC5, Interleukin 15 Receptor Alpha Isoform IC6, Interleukin 15 Receptor Alpha Isoform IC7,Interleukin 15 Receptor Alpha Isoform IC8, Interleukin-15 Receptor Subunit Alpha,� IL-15 Receptor Subunit Alpha, CD215 Antigen, IL-15R-Alpha, IL-15RA, CD215.
Source:Sf9 Insect cells
Physical Appearance:Sterile filtered colorless solution.
Formulation:IL15RA protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPITCPPPM SVEHADIWVK SYSLYSRERY ICNSGFKRKA GTSSLTECVL NKATNVAHWT TPSLKCIRDP ALVHQRPAPP STVTTAGVTP QPESLSPSGK EPAASSPSSN NTAATTAAIV PGSQLMPSKS PSTGTTEISS HESSHGTPSQ TTAKNWELTA SASHQPPGVY PQGHSDTTLE�PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP�VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
Biological Activity:Determined by its ability to inhibit proliferation using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2 ng/ml with Human IL-15.

IL15RA, also known as Interleukin 15 Receptor Alpha, is a high-affinity receptor for interleukin-15. IL15RA plays a role as a heterotrimer with the IL-2 receptor beta and gamma subunits to commence signal transduction. IL15/IL15R alpha complexes can transmit reverse signaling which promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, as well as cytokine secretion by the IL?15/IL15R alpha expressing cells.

IL15RA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (31-205 a.a.) and fused to a 242 a.a. hIgG-His Tag at C-terminus containing a total of 417 amino acids and having a molecular mass of 45.6kDa.IL15RA shows multiple bands between 57-70kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

UniProt Protein Function:IL15RA: High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 and SYK. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: plasma membrane

Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; signal transducer activity

Biological Process: cell proliferation; signal transduction

NCBI Summary:This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq, Apr 2010]
UniProt Code:Q13261
NCBI GenInfo Identifier:59799763
NCBI Gene ID:3601
NCBI Accession:Q13261.1
UniProt Secondary Accession:Q13261,Q3B769, Q5JVA1, Q5JVA2, Q5JVA4, Q6B0J2, Q7LDR4 Q7Z609, B4E2C2,
UniProt Related Accession:Q13261
Molecular Weight:24,388 Da
NCBI Full Name:Interleukin-15 receptor subunit alpha
NCBI Synonym Full Names:interleukin 15 receptor subunit alpha
NCBI Official Symbol:IL15RA��
NCBI Official Synonym Symbols:CD215��
NCBI Protein Information:interleukin-15 receptor subunit alpha
UniProt Protein Name:Interleukin-15 receptor subunit alpha
UniProt Synonym Protein Names:CD_antigen: CD215
Protein Family:Interleukin-15 receptor
UniProt Gene Name:IL15RA��
UniProt Entry Name:I15RA_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13261
Research Area:
Cytokines
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