Cytokines Recombinant Proteins

Human IL 3 Recombinant Protein (RPPB0497)

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SKU:
RPPB0497
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P08700
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human IL 3 Recombinant Protein
Product Code:RPPB0497
Size:10µg
Species:Human
Target:IL 3
Synonyms:MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Source:Insect Cells
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5X PBS pH-7.4.
Solubility:It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr
Biological Activity:The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg.

IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Interleukin-3 Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.

UniProt Protein Function:IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.
UniProt Protein Details:

Protein type:Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine; Oncoprotein; Apoptosis

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function:interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of tyrosine phosphorylation of Stat5 protein; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication

NCBI Summary:The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
UniProt Code:P08700
NCBI GenInfo Identifier:124330
NCBI Gene ID:3562
NCBI Accession:P08700.2
UniProt Secondary Accession:P08700,Q6GS87,
UniProt Related Accession:P08700
Molecular Weight:17,233 Da
NCBI Full Name:Interleukin-3
NCBI Synonym Full Names:interleukin 3
NCBI Official Symbol:IL3��
NCBI Official Synonym Symbols:IL-3; MCGF; MULTI-CSF��
NCBI Protein Information:interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; colony-stimulating factor, multiple; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
UniProt Protein Name:Interleukin-3
UniProt Synonym Protein Names:Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
Protein Family:Interleukin
UniProt Gene Name:IL3��
UniProt Entry Name:IL3_HUMAN
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