Human IL-27 PharmaGenie ELISA Kit (SBRS0765)
- SKU:
- SBRS0765
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Human IL-27 PharmaGenie ELISA Kit (SBRS0765)
| Product SKU: | SBRS0765 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, DLL1, Fetuin A, FOLR1, GASP-1, GASP-2, G-CS R, HAI-2, IL-17B R, LAG-3, LDL R, Pepsinogen I, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin-1, TRANCE, Troponin I, uPA, WISP-1. |
| Uniprot: | Q8NEV9 |
| Gene ID: | 246778 |
| Gene Names: | IL27 / IL27A / IL30 |
| Synonyms: | Interleukin-27 subunit alpha (IL-27 subunit alpha) (IL-27-A) (IL27-A) (Interleukin-30) (p28) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 81 pg/ml - 20,000 pg/ml |
| Sensitivity: | 81 pg/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human IL-27 PharmaGenie ELISA Kit (SBRS0765) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
Related Products
Human IL-27 R alpha PharmaGenie ELISA Kit (SBRS0766)
system_update_altDatasheetHuman IL-27 R alpha PharmaGenie ELISA Kit (SBRS0766)Overview
