Cytokines Recombinant Proteins

Human il 18 Recombinant Protein (RPPB0620)

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RPPB0620

Description

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Product Name:Human il 18 Recombinant Protein
Product Code:RPPB0620
Size:25µg
Species:Human
Target:il 18
Synonyms:IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a concentrated (1mg/ml) solution in PBS and DTT.
Solubility:It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18M?-cm H2O not less than 200�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Biological Activity:The ED50 is 1.5-9 ng/mL and is measured by Interleukin 18 ability to induce IFN-? secretion by KG?1 human acute myelogenous leukemia cells in the presence of TNF-alpha.

IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 18.2 kDa. The IL-18 is purified by proprietary chromatographic techniques.

UniProt Protein Function:IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.
UniProt Protein Details:

Protein type:Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function:protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response

NCBI Summary:The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Code:Q14116
NCBI GenInfo Identifier:3219817
NCBI Gene ID:3606
NCBI Accession:Q14116.1
UniProt Secondary Accession:Q14116,O75599, Q6FGY3, Q6WWJ7,
UniProt Related Accession:Q14116
Molecular Weight:21,896 Da
NCBI Full Name:Interleukin-18
NCBI Synonym Full Names:interleukin 18
NCBI Official Symbol:IL18��
NCBI Official Synonym Symbols:IGIF; IL-18; IL-1g; IL1F4��
NCBI Protein Information:interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
UniProt Protein Name:Interleukin-18
UniProt Synonym Protein Names:Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family:Interleukin
UniProt Gene Name:IL18��
UniProt Entry Name:IL18_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q14116
Research Area:
Cytokines
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