Cytokines Recombinant Proteins

Human IL 12 p35 Recombinant Protein (RPPB0566)

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RPPB0566

Description

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Product Name:Human IL 12 p35 Recombinant Protein
Product Code:RPPB0566
Size:10µg
Species:Human
Target:IL 12 p35
Synonyms:NKSF1, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 35 kDa subunit (CLMF p35), TSF, Edodekin-alpha, IL-12 p35, IL-12A, IL-12 subunit p35, NK cell stimulatory factor chain 1, SGT2, FLJ39002.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:Interleukin-12 p35 His-Tag is supplied in 1xPBS and 50% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.

Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.

Interleukin-12 p35 His Human Recombinant produced in E.Coli is single, a non-glycosylated, polypeptide chain containing 197 amino acids fragment (57-253) with an amino-terminal hexahistidine tag. The IL-12 p35 His is purified by proprietary chromatographic techniques.

UniProt Protein Function:IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: extracellular region; extracellular space; interleukin-12 complex

Molecular Function:cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity

Biological Process: cell cycle arrest; cell migration; defense response to Gram-positive bacterium; immune response; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; positive regulation of cell adhesion; positive regulation of interferon-gamma production; positive regulation of lymphocyte proliferation; positive regulation of mononuclear cell proliferation; positive regulation of natural killer cell activation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of NK T cell activation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; response to UV-B; response to virus

NCBI Summary:This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
UniProt Code:P29459
NCBI GenInfo Identifier:20141534
NCBI Gene ID:3592
NCBI Accession:P29459.2
UniProt Secondary Accession:P29459,Q96QZ1,
UniProt Related Accession:P29459
Molecular Weight:24,874 Da
NCBI Full Name:Interleukin-12 subunit alpha
NCBI Synonym Full Names:interleukin 12A
NCBI Official Symbol:IL12A��
NCBI Official Synonym Symbols:P35; CLMF; NFSK; NKSF1; IL-12A��
NCBI Protein Information:interleukin-12 subunit alpha
UniProt Protein Name:Interleukin-12 subunit alpha
UniProt Synonym Protein Names:Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
Protein Family:Interleukin
UniProt Gene Name:IL12A��
UniProt Entry Name:IL12A_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P29459
Research Area:
Cytokines
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