Growth Factors & Cytokines Recombinant Proteins

Human IGFBP 4 Recombinant Protein (RPPB0433)

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RPPB0433

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human IGFBP 4 Recombinant Protein
Product Code:RPPB0433
Size:20µg
Species:Human
Target:IGFBP 4
Synonyms:Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP.
Source:HEK293 Cells
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:IGFBP 4 was lyophilized after extensive dialysis against PBS.
Solubility:It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag
Biological Activity:The ED50 range is 0.01-0.09�g/ml�as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.

Insulin-like growth factor-binding protein 4 (IGFBP-4) belongs to the insulin-like growth factor binding protein (IGFBP) family. IGFBP4 includes an IGFBP domain and a thyroglobulin type-I domain. IGFBP4 binds both insulin-like growth factors (IGFs) I and II. IGFBP-4 circulates in the plasma in both glycosylated and non-glycosylated forms. IGFBPs can either inhibit or enhance the biological activities of IGF, or act in an IGF independent manner. IGFBP-4 is exceptional since it consistently inhibits several cancer cells in vivo and in vitro, suggesting that it may function as an apoptotic factor. IGFBP4 is produced by all colon cancer cells. Binding of IGFBP-4 prolongs the half-life of the IGFs and changes their interaction with cell surface receptors.

IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions.IGFBP4 is purified by proprietary chromatographic techniques.

UniProt Protein Function:IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: extracellular region; extracellular space

Molecular Function:insulin-like growth factor I binding; insulin-like growth factor II binding; receptor binding

Biological Process: cell proliferation; cellular protein metabolic process; DNA metabolic process; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; skeletal development

NCBI Summary:This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
UniProt Code:P22692
NCBI GenInfo Identifier:124065
NCBI Gene ID:3487
NCBI Accession:P22692.2
UniProt Secondary Accession:P22692,Q5U012, Q9UCL6, A0N9W2, B4E351,
UniProt Related Accession:P22692
Molecular Weight:17,635 Da
NCBI Full Name:Insulin-like growth factor-binding protein 4
NCBI Synonym Full Names:insulin like growth factor binding protein 4
NCBI Official Symbol:IGFBP4��
NCBI Official Synonym Symbols:BP-4; IBP4; IGFBP-4; HT29-IGFBP��
NCBI Protein Information:insulin-like growth factor-binding protein 4
UniProt Protein Name:Insulin-like growth factor-binding protein 4
Protein Family:Insulin-like growth factor-binding protein
UniProt Gene Name:IGFBP4��
UniProt Entry Name:IBP4_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22692
Research Area:
Growth Factors & Cytokines
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