Human IF Recombinant Protein (RPPB3753)

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RPPB3753

Description

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Product Name:Human IF Recombinant Protein
Product Code:RPPB3753
Size:10µg
Species:Human
Target:IF
Synonyms:Gastric intrinsic factor, Intrinsic factor, INF, IF, GIF, IFMH, TCN3, Cobalamin/Vitamin B-12 binding transport protein.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered pink solution.
Formulation:The protein solution contains 20mM HEPES pH-8.0, 100mM NaCl�and 20% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Intrinsic Factor is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for erythrocyte maturation and mutations in the Intrinsic Factor may lead to congenital pernicious anemia. Upon entry into the stomach, vitamin B12 binds to one of two B12 binding proteins present in the gastric fluid. In the less acidic environment of the small intestine, these proteins dissociate from the vitamin, allowing it to bind to intrinsic factor and enter the portal circulation through a receptor in the ileal mucosa specific for the B12-intrinsic factor complex.

Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton. The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.

UniProt Protein Function:GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; lysosomal lumen; microvillus; apical plasma membrane; extracellular region; endosome

Molecular Function:cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process

Disease: Intrinsic Factor Deficiency

NCBI Summary:This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008]
UniProt Code:P27352
NCBI GenInfo Identifier:62906845
NCBI Gene ID:2694
NCBI Accession:P27352.2
UniProt Related Accession:P27352
Molecular Weight:
NCBI Full Name:Cobalamin binding intrinsic factor
NCBI Synonym Full Names:cobalamin binding intrinsic factor
NCBI Official Symbol:CBLIF��
NCBI Official Synonym Symbols:IF; GIF; INF; IFMH; TCN3��
NCBI Protein Information:cobalamin binding intrinsic factor
UniProt Protein Name:Gastric intrinsic factor
UniProt Synonym Protein Names:Intrinsic factor; IF; INF
Protein Family:Gastric intrinsic factor
UniProt Gene Name:GIF��
UniProt Entry Name:IF_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P27352
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