Human ICT1 Recombinant Protein (RPPB3732)

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SKU:
RPPB3732
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q14197

Description

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Product Name:Human ICT1 Recombinant Protein
Product Code:RPPB3732
Size:20µg
Species:Human
Target:ICT1
Synonyms:DS-1, DS1, Peptidyl-tRNA hydrolase ICT1 mitochondrial, Digestion substraction 1, Immature colon carcinoma transcript 1 protein, ICT1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:ICT1 protein solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 30% glycerol, 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSLHKQKDG TEFKSIYSLD KLYPESQGSD TAWRVPNGAK QADSDIPLDR LTISYCRSSG PGGQNVNKVN SKAEVRFHLA TAEWIAEPVR QKIAITHKNK INRLGELILT SESSRYQFRN LADCLQKIRD MITEASQTPK EPTKEDVKLH RIRIENMNRE RLRQKRIHSA VKTSRRVDMD

Immature Colon Carcinoma Transcript 1 (ICT1) functions as a codon-independent translation release factor that has lost all stop codon specificity and leads the termination of translation in mitochondrion in case of abortive elongation. The mature colon epithelium has 3 differentiated cell types which arise from a multipotent stem cell. ICT1 is involved in the hydrolysis of peptidyl-tRNAs which have been prematurely terminated and consequently in the recycling of stalled mitochondrial ribosomes. Digression from the normal maturation pathway by neoplastic transformation is assumed to originate in stem cells or their early descendants.

ICT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a) and having a molecular mass of 22.8kDa. ICT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.
UniProt Protein Details:

Protein type:EC 3.1.1.29; Mitochondrial

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial matrix; mitochondrion

Molecular Function:aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific

NCBI Summary:The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:Q14197
NCBI GenInfo Identifier:6015033
NCBI Gene ID:3396
NCBI Accession:Q14197.1
UniProt Secondary Accession:Q14197,Q53HM7, Q53Y11, B2RAD1,
UniProt Related Accession:Q14197
Molecular Weight:24kDa
NCBI Full Name:Peptidyl-tRNA hydrolase ICT1, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L58
NCBI Official Symbol:MRPL58��
NCBI Official Synonym Symbols:DS1; DS-1; ICT1; MRP-L58��
NCBI Protein Information:peptidyl-tRNA hydrolase ICT1, mitochondrial
UniProt Protein Name:Peptidyl-tRNA hydrolase ICT1, mitochondrial
UniProt Synonym Protein Names:39S ribosomal protein L58, mitochondrial
Protein Family:1-acylglycerol-3-phosphate O-acyltransferase
UniProt Gene Name:MRPL58��
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