Human IBSP Recombinant Protein (RPPB3723)
- SKU:
- RPPB3723
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P21815
Description
| Product Name: | Human IBSP Recombinant Protein |
| Product Code: | RPPB3723 |
| Size: | 10µg |
| Species: | Human |
| Target: | IBSP |
| Synonyms: | Integrin Binding Sialoprotein, Integrin-Binding Sialoprotein,Bone Sialoprotein II, Cell-Binding Sialoprotein, BSP II , BNSP, BoneSialoprotein, BSP-II, SP-II, BSP. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IBSP protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4), 10% glycerol and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | FSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDSSEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKAGDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESVTGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVYRT TSPPFGKTTT VEYEGEYEYT GANEYDNGYEIYESENGEPR GDNYRAYEDE YSYFKGQGYD GYDGQNYYHH QLEHHHHHH |
IntegrinBinding Sialoprotein (IBSP) is a monomeric noncollagenous protein which belongsto the SIBLING family of extracellular matrix (ECM) proteins. IBSP issynthesized by platelets, odontoblasts, osteocytes, osteoblasts, osteoclasts,and bone marrow stromal cells. IBSP forms an integral part of the mineralizedmatrix and also plays a role as the main mediator of the hypertrophicchondrocytes-induced angiogenesis. IBSP which binds firmly to hydroxyapatite isrelated to the severity of histological cartilage lesions.
IBSP produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 309 amino acids (17-317aa) and havinga molecular mass of 34.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducingconditions).�IBSP is expressed with a 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: extracellular space; membrane; extracellular region Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion |
| NCBI Summary: | The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P21815 |
| NCBI GenInfo Identifier: | 317373545 |
| NCBI Gene ID: | 3381 |
| NCBI Accession: | P21815.4 |
| UniProt Related Accession: | P21815 |
| Molecular Weight: | 35,148 Da |
| NCBI Full Name: | Bone sialoprotein 2 |
| NCBI Synonym Full Names: | integrin-binding sialoprotein |
| NCBI Official Symbol: | IBSP�� |
| NCBI Official Synonym Symbols: | BSP; BNSP; SP-II; BSP-II�� |
| NCBI Protein Information: | bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein |
| UniProt Protein Name: | Bone sialoprotein 2 |
| UniProt Synonym Protein Names: | Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein |
| Protein Family: | Bone sialoprotein |
| UniProt Gene Name: | IBSP�� |
| UniProt Entry Name: | SIAL_HUMAN |
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