Enzymes Recombinant Proteins

Human HSD17B8 Recombinant Protein (RPPB1835)

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SKU:
RPPB1835
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q92506
Research Area:
Enzymes

Description

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Product Name:Human HSD17B8 Recombinant Protein
Product Code:RPPB1835
Size:20µg
Species:Human
Target:HSD17B8
Synonyms:E2 17-beta-dehydrogenase 8, 17-beta-hydroxysteroid dehydrogenase 8, 17-beta-HSD 8, 3-oxoacyl-[acyl-carrier-protein] reductase, Protein Ke6, Ke-6, Really interesting new gene 2 protein, Testosterone 17-beta-dehydrogenase 8, HSD17B8, FABGL, HKE6, RING2, KE6, FABG, H2-KE6, SDR30C1, D6S2245E, dJ1033B10.9.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:HSD17B8 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol and 150mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITG TSVEVTGGLF M

E2 17-beta-dehydrogenase 8 (HSD17B8) is a member of the short-chain dehydrogenases/reductases (SDR) family. In mice, the HSD17B8 protein is a 17-beta-hydroxysteroid dehydrogenase which can regulate the concentration of biologically active estrogens and androgens. HSD17B8 is preferentially an oxidative enzyme and inactivates E2, testosterone, and DHT. However, the HSD17B8 enzyme has some reductive activity and can synthesize E2 from E1. HSD17B8 may have a role in biosynthesis of fatty acids in mitochondria.

HSD17B8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 29.1kDa.HSD17B8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HSD17B8: NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3- ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:

Protein type:Lipid Metabolism - androgen and estrogen; EC 1.1.1.239; Mitochondrial; EC 1.1.1.62; Oxidoreductase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: mitochondrial matrix

Molecular Function:3-hydroxyacyl-CoA dehydrogenase activity; estradiol 17-beta-dehydrogenase activity; protein binding

Biological Process: estrogen biosynthetic process

NCBI Summary:In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q92506
NCBI GenInfo Identifier:12643402
NCBI Gene ID:7923
NCBI Accession:Q92506.2
UniProt Secondary Accession:Q92506,Q5STP7, Q9UIQ1, A6NLX7,
UniProt Related Accession:Q92506
Molecular Weight:26,974 Da
NCBI Full Name:Estradiol 17-beta-dehydrogenase 8
NCBI Synonym Full Names:hydroxysteroid 17-beta dehydrogenase 8
NCBI Official Symbol:HSD17B8��
NCBI Official Synonym Symbols:KE6; FABG; HKE6; FABGL; RING2; H2-KE6; SDR30C1; D6S2245E; dJ1033B10.9��
NCBI Protein Information:estradiol 17-beta-dehydrogenase 8
UniProt Protein Name:Estradiol 17-beta-dehydrogenase 8
UniProt Synonym Protein Names:17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-); Protein Ke6; Ke-6; Really interesting new gene 2 protein; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8 (EC:1.1.1.239)
Protein Family:Estradiol 17 beta-dehydrogenase
UniProt Gene Name:HSD17B8��
UniProt Entry Name:DHB8_HUMAN
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