Enzymes Recombinant Proteins

Human HPGDS Recombinant Protein (RPPB5816)

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SKU:
RPPB5816
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O60760
Research Area:
Enzymes

Description

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Product Name:Human HPGDS Recombinant Protein
Product Code:RPPB5816
Size:20µg
Species:Human
Target:HPGDS
Synonyms:Hematopoietic prostaglandin D synthase, H-PGDS, GST class-sigma, Glutathione S-transferase, Glutathione-dependent PGD synthase, Glutathione-requiring prostaglandin D synthase, Prostaglandin-H2 D-isomerase, HPGDS, GSTS, PGDS, PTGDS2GSTS, PGD2, GSTS1-1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:HPGDS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMPNYKL TYFNMRGRAE IIRYIFAYLD IQYEDHRIEQ ADWPEIKSTL PFGKIPILEV DGLTLHQSLA IARYLTKNTD LAGNTEMEQC HVDAIVDTLD DFMSCFPWAE KKQDVKEQMF NELLTYNAPH LMQDLDTYLG GREWLIGNSV TWADFYWEIC STTLLVFKPD LLDNHPRLVT LRKKVQAIPA VANWIKRRPQ TKL

Hematopoietic Prostaglandin D Synthase (HPGDS) belongs to the sigma class glutathione-S-transferase family. The HPGDS enzyme catalyzes the conversion of PGH2 to PGD2 and has a role in the production of prostanoids in the immune system and mast cells. The presence of the HPGDS enzyme can be utilized to identify the differentiation stage of human megakaryocytes. Furthermore, HPGDS is a prostaglandin involved in smooth muscle contraction/relaxation and an effective inhibitor of platelet aggregation, and the conjugation of glutathione with a extensive range of aryl halides and organic isothiocyanates. In addition, HPGDS displays low glutathione-peroxidase activity towards cumene hydroperoxide. HPGDS is expressed in a number of megakaryocytic cell lines but not in platelets. HPGDS is highly expressed in the adipose tissue, macrophages and placenta; however it is expressed at lower levels in the lung, heart, lymph nodes, appendix, bone marrow and fetal liver.

HPGDS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-199 a.a.) and having a molecular mass of 25.9kDa.HPGDS is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Ptgds2: Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide. Belongs to the GST superfamily. Sigma family.
UniProt Protein Details:

Protein type:EC 2.5.1.18; Lipid Metabolism - arachidonic acid; EC 5.3.99.2; Isomerase

Chromosomal Location of Human Ortholog: 4q22.3

Cellular Component: cytoplasm; cytosol

Molecular Function:prostaglandin-D synthase activity; protein homodimerization activity; glutathione transferase activity; magnesium ion binding; calcium ion binding

Biological Process: xenobiotic metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; locomotory behavior; signal transduction; prostaglandin metabolic process

NCBI Summary:Prostaglandin-D synthase is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells. The presence of this enzyme can be used to identify the differentiation stage of human megakaryocytes. [provided by RefSeq, Jul 2008]
UniProt Code:O60760
NCBI GenInfo Identifier:49456319
NCBI Gene ID:27306
NCBI Accession:CAG46480.1
UniProt Related Accession:O60760
Molecular Weight:~ 23kDa
NCBI Full Name:PGDS, partial
NCBI Synonym Full Names:hematopoietic prostaglandin D synthase
NCBI Official Symbol:HPGDS��
NCBI Official Synonym Symbols:GSTS; PGD2; PGDS; GSTS1; GSTS1-1��
NCBI Protein Information:hematopoietic prostaglandin D synthase
UniProt Protein Name:Hematopoietic prostaglandin D synthase
UniProt Synonym Protein Names:GST class-sigma; Glutathione S-transferase (EC:2.5.1.18); Glutathione-dependent PGD synthase; Glutathione-requiring prostaglandin D synthase; Prostaglandin-H2 D-isomerase
UniProt Gene Name:HPGDS��
UniProt Entry Name:HPGDS_HUMAN
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