Human HNRNPA1 Recombinant Protein (RPPB5894)

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RPPB5894

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Product Name:Human HNRNPA1 Recombinant Protein
Product Code:RPPB5894
Size:20µg
Species:Human
Target:HNRNPA1
Synonyms:Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1, HNRNPA1, HNRPA1, HNRPA1L3, hnRNP-A1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:HNRNPA1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSKSESP KEPEQLRKLF IGGLSFETTD ESLRSHFEQW GTLTDCVVMR DPNTKRSRGF GFVTYATVEE VDAAMNARPH KVDGRVVEPK RAVSREDSQR PGAHLTVKKI FVGGIKEDTE EHHLRDYFEQ YGKIEVIEIM TDRGSGKKRG FAFVTFDDHD SVDKIVIQKY HTVNGHNCEV RKALSKQEMA SASSSQRGRS GSGNFGGGRG GGFGGNDNFG RGGNFSGRGG FGGSRGGGGY GGSGDGYNGF GNDGSNFGGG GSYNDFGNYN NQSSNFGPMK GGNFGGRSSG PYGGGGQYFA KPRNQGGYGG SSSSSSYGSG RRF

Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) is a member of the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). HNRNPA1 is involved in the packing of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may control splice site selection. The HNRNPA1 protein may also have a role in HCV RNA replication. In addition, HNRNPA1 is believed to have a key role in the formation of specific myometrial protein species in parturition. The hnRNP proteins have distinctive nucleic acid binding properties. HNRNPA1 has 2 repeats of quasi-RRM domains that bind to RNAs. HNRNPA1 is one of the most copious core proteins of hnRNP complexes and it is restricted to the nucleoplasm. The HNRNPA1 protein, along with other hnRNP proteins, is exported from the nucleus, most likely bound to mRNA, and is immediately re-imported. The M9 domain of HNRNPA1 functions as both a nuclear localization and nuclear export signal.

HNRNPA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-320 a.a.) and having a molecular mass of 36.6kDa.HNRNPA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:hnRNP A1: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; RNA splicing; Spliceosome; Nuclear export

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; ribonucleoprotein complex; spliceosome

Molecular Function:protein binding; RNA binding; single-stranded DNA binding; single-stranded RNA binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA processing; negative regulation of telomere maintenance via telomerase; nuclear export; nuclear import; nuclear mRNA splicing, via spliceosome; positive regulation of telomere maintenance via telomerase; RNA export from nucleus

Disease: Amyotrophic Lateral Sclerosis 20; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 3

NCBI Summary:This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]
UniProt Code:P09651
NCBI GenInfo Identifier:288558857
NCBI Gene ID:3178
NCBI Accession:P09651.5
UniProt Secondary Accession:P09651,Q3MIB7, Q6PJZ7, A8K4Z8,
UniProt Related Accession:P09651
Molecular Weight:29,386 Da
NCBI Full Name:Heterogeneous nuclear ribonucleoprotein A1
NCBI Synonym Full Names:heterogeneous nuclear ribonucleoprotein A1
NCBI Official Symbol:HNRNPA1��
NCBI Official Synonym Symbols:UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1��
NCBI Protein Information:heterogeneous nuclear ribonucleoprotein A1
UniProt Protein Name:Heterogeneous nuclear ribonucleoprotein A1
UniProt Synonym Protein Names:Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1
Protein Family:Heterogeneous nuclear ribonucleoproteins
UniProt Gene Name:HNRNPA1��
UniProt Entry Name:ROA1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P09651
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