Description
| Product Name: | Human HNMT Recombinant Protein |
| Product Code: | RPPB1819 |
| Size: | 5µg |
| Species: | Human |
| Target: | HNMT |
| Synonyms: | �HMT, HNMT-S1, HNMT-S2, MRT51. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | HNMT protein solution (1mg/ml) containing 20mM, Tris-Hcl (pH8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA |
| Biological Activity: | Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37�C. |
Histamine N-Methyltransferase or HNMT, is located in the cell cytosol. Sadenosyl-L-methionine acts as a methyl donor for HNMT which inactivates histamine. By inactivation of histamine, it affects the immune system�s response. HNMT acts on histamine in body tissues such as kidney, central nervous system and bronchus. The protein has a crucial part in the airway response to histamine & histamine degradation process and regulation.
HNMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 328 amino acids (1-292 a.a) and having a molecular mass of 37.4kDa. HNMT is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HNMT: Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. Belongs to the methyltransferase superfamily. HNMT family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - histidine; EC 2.1.1.8; Methyltransferase Chromosomal Location of Human Ortholog: 2q22.1 Cellular Component: cytoplasm; cytosol; neuron projection; nucleoplasm Molecular Function:histamine N-methyltransferase activity Biological Process: brain development; histidine catabolic process; methylation; respiratory gaseous exchange Disease: Asthma, Susceptibility To |
| NCBI Summary: | In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50135 |
| NCBI GenInfo Identifier: | 1708272 |
| NCBI Gene ID: | 3176 |
| NCBI Accession: | P50135.1 |
| UniProt Secondary Accession: | P50135,Q546Z6, Q7Z7I2, Q8IU56, Q8WW98, Q9BRW6, B2R9J3 |
| UniProt Related Accession: | P50135 |
| Molecular Weight: | 6,046 Da |
| NCBI Full Name: | Histamine N-methyltransferase |
| NCBI Synonym Full Names: | histamine N-methyltransferase |
| NCBI Official Symbol: | HNMT�� |
| NCBI Official Synonym Symbols: | HMT; MRT51; HNMT-S1; HNMT-S2�� |
| NCBI Protein Information: | histamine N-methyltransferase |
| UniProt Protein Name: | Histamine N-methyltransferase |
| Protein Family: | Histamine N-methyltransferase |
| UniProt Gene Name: | HNMT�� |
| UniProt Entry Name: | HNMT_HUMAN |
