Human HIF-1 alpha PharmaGenie ELISA Kit (SBRS0675)
- SKU:
- SBRS0675
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
HIF-1 alpha ELISA
HIF1A (Hypoxia Inducible Factor 1 Subunit Alpha) is a protein that is expressed in response to low oxygen levels. It has been shown to play a role in tumorigenesis and metastasis. HIF1A is involved in the process of angiogenesis, which is the formation of new blood vessels. HIF1A also plays a role in the regulation of energy metabolism. Diseases associated with HIF1A include Hypoxia and Retinal Ischemia. Among its related pathways are Signaling by PTK6 and CDK-mediated phosphorylation and removal of Cdc6.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | HIF-1 alpha ELISA Kit |
| Product Code: | HUFI00398 |
| Size: | 96 Assays |
| Alias: | HIF-1 alpha, HIF1A, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1-alpha, hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, PAS domain-containing protein 8 |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human HIF1A concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.094ng/ml |
| Range: | 0.156-10ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery: | Matrices listed below were spiked with certain level of Human HIF1A and the recovery rates were calculated by comparing the measured value to the expected amount of Human HIF1A in samples.
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human HIF1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%): | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 |
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