Human HEXIM1 Recombinant Protein (RPPB3676)

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SKU:
RPPB3676
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O94992

Description

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Product Name:Human HEXIM1 Recombinant Protein
Product Code:RPPB3676
Size:10µg
Species:Human
Target:HEXIM1
Synonyms:CLP1, EDG1, HIS1, MAQ1, Protein HEXIM1, Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, Cardiac lineage protein 1, Menage a quatre protein 1, FLJ13562.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:HEXIM1 solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 0.1M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD

HEXIM1 expression is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. HEXIM1 is a transcriptional regulator which acts as a universal RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex inhibits RNA polymerase II phosphorylation following transcriptional elongation. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

HEXIM1 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 396 amino acids (1-359a.a.) and having a molecular mass of 44.8 kDa. HEXIM1 is fused to a 37 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.
UniProt Protein Details:

Protein type:Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding

Biological Process: heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent

NCBI Summary:Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]
UniProt Code:O94992
NCBI GenInfo Identifier:74735573
NCBI Gene ID:10614
NCBI Accession:O94992.1
UniProt Secondary Accession:O94992,B2R8Y5,
UniProt Related Accession:O94992
Molecular Weight:40,623 Da
NCBI Full Name:Protein HEXIM1
NCBI Synonym Full Names:hexamethylene bis-acetamide inducible 1
NCBI Official Symbol:HEXIM1��
NCBI Official Synonym Symbols:CLP1; EDG1; HIS1; MAQ1��
NCBI Protein Information:protein HEXIM1
UniProt Protein Name:Protein HEXIM1
UniProt Synonym Protein Names:Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
Protein Family:Protein
UniProt Gene Name:HEXIM1��
UniProt Entry Name:HEXI1_HUMAN
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