Enzymes Recombinant Proteins

Human HDDC3 Recombinant Protein (RPPB1804)

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SKU:
RPPB1804
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8N4P3
Research Area:
Enzymes

Description

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Product Name:Human HDDC3 Recombinant Protein
Product Code:RPPB1804
Size:20µg
Species:Human
Target:HDDC3
Synonyms:Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, Metazoan SpoT homolog 1, MESH1, Penta-phosphate guanosine-3'-pyrophosphohydrolase, (ppGpp)ase, HDDC3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The HDDC3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ

Guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1 (HDDC3) contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. In accordance with its structure, HDDC3 effectively catalyzes the hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. In addition, HDDC3 suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria.

HDDC3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (1-140) and having a molecular mass of 17.9kDa.HDDC3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HDDC3: ppGpp hydrolyzing enzyme involved in starvation response. Belongs to the MESH1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.7.2Chromosomal Location of Human Ortholog: 15q26.1Molecular Function: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; metal ion bindingBiological Process: metabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q8N4P3
NCBI GenInfo Identifier:38348360
NCBI Gene ID:374659
NCBI Accession:NP_940929.1
UniProt Secondary Accession:Q8N4P3
UniProt Related Accession:Q8N4P3
Molecular Weight:
NCBI Full Name:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 isoform 2
NCBI Synonym Full Names:HD domain containing 3
NCBI Official Symbol:HDDC3��
NCBI Official Synonym Symbols:MESH1; (ppGpp)ase��
NCBI Protein Information:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
UniProt Protein Name:Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
UniProt Synonym Protein Names:HD domain-containing protein 3; Metazoan SpoT homolog 1; MESH1; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase
Protein Family:
UniProt Gene Name:HDDC3��
UniProt Entry Name:MESH1_HUMAN
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