Chemokines Recombinant Proteins

Human HCC 1 Recombinant Protein (RPPB1157)

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SKU:
RPPB1157
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16627
Research Area:
Chemokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human HCC 1 Recombinant Protein
Product Code:RPPB1157
Size:10µg
Species:Human
Target:HCC 1
Synonyms:Small inducible cytokine A14, CCL14, Chemokine CC-1/CC-3, HCC-1/HCC-3, HCC-1(1-74), NCC-2, chemokine (C-C motif) ligand 14, CC-1, CC-3, CKb1, MCIF, SY14, HCC-1, HCC-3, SCYL2, SCYA14.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The CCL14 protein was lyophilized with 20mM PBS pH-7.4 and 150mM NaCl.
Solubility:It is recommended to reconstitute the lyophilized HCC-1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized HCC1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:TESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRGHSVCTNPSDKWVQDYIKDMKEN
Biological Activity:The Biological activity is calculated by its ability to chemoattract Human monocytes at 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is procesed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL13 activates monocytes, but does not induce their chemotaxis. Human CCL13 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

HCC-1 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 8411 Dalton. The HCC-1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function:chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response

NCBI Summary:This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]
UniProt Code:Q16627
NCBI GenInfo Identifier:14589959
NCBI Gene ID:6358
NCBI Accession:NP_116738.1
UniProt Secondary Accession:Q16627,Q13954, E1P649, E1P650,
UniProt Related Accession:Q16627
Molecular Weight:~9.5 kDa
NCBI Full Name:C-C motif chemokine 14 isoform 2
NCBI Synonym Full Names:chemokine (C-C motif) ligand 14
NCBI Official Symbol:CCL14��
NCBI Official Synonym Symbols:CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3��
NCBI Protein Information:C-C motif chemokine 14
UniProt Protein Name:C-C motif chemokine 14
UniProt Synonym Protein Names:Chemokine CC-1/CC-3; HCC-1/HCC-3
Protein Family:C-C motif chemokine
UniProt Gene Name:CCL14��
UniProt Entry Name:CCL14_HUMAN
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