Growth Factors & Cytokines Recombinant Proteins

Human HB-EGF Recombinant Protein (RPPB0272)

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SKU:
RPPB0272
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99075
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human HB-EGF Recombinant Protein
Product Code:RPPB0272
Size:10µg
Species:Human
Target:HB-EGF
Synonyms:Proheparin-binding EGF-like growth factor, HBEGF, DTR, DTS, HEGFL, HB-EGF, Heparin-binding EGF-like growth factor, Diphtheria toxin receptor, DT-R, DTSF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The HB-EGF His solution (0. 5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 50% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSDLQEADL DLLRVTLSSK PQALATPNKE EHGKRKKKGK GLGKKRDPCL RKYKDFCIHG ECKYVKELRA PSCICHPGYH GERCHGLSL

HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF�s ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.

HB-EGF His Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (63-148) and having a molecular mass of 12.1kDa.HB-EGF His is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function:heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; positive regulation of cell migration; wound healing, spreading of epidermal cells

UniProt Code:Q99075
NCBI GenInfo Identifier:544477
NCBI Gene ID:1839
NCBI Accession:Q99075.1
UniProt Secondary Accession:Q99075,B2R821,
UniProt Related Accession:Q99075
Molecular Weight:Calculated MW: 23kDaObserved MW: 23kDa
NCBI Full Name:Proheparin-binding EGF-like growth factor
NCBI Synonym Full Names:heparin-binding EGF-like growth factor
NCBI Official Symbol:HBEGF��
NCBI Official Synonym Symbols:DTR; DTS; DTSF; HEGFL��
NCBI Protein Information:proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
UniProt Protein Name:Proheparin-binding EGF-like growth factor
UniProt Synonym Protein Names:Diphtheria toxin receptor; DT-R
Protein Family:Proheparin-binding EGF-like growth factor
UniProt Gene Name:HBEGF��
UniProt Entry Name:HBEGF_HUMAN
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