Enzymes Recombinant Proteins

Human HARS Recombinant Protein (RPPB1798)

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RPPB1798

Description

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Product Name:Human HARS Recombinant Protein
Product Code:RPPB1798
Size:25µg
Species:Human
Target:HARS
Synonyms:Histidyl-tRNA synthetase, EC 6.1.1.21, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).
Stability:Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Histidyl-tRNA Synthetase Human Recombinant produced in baculovirus is a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa.The Histidyl-tRNA Synthetase is fused to 6x His Tag and purified by proprietary chromatographic techniques.

UniProt Protein Function:HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:EC 6.1.1.21; Translation; Ligase

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function:histidine-tRNA ligase activity

Biological Process: histidyl-tRNA aminoacylation; mitochondrial translation; tRNA aminoacylation for protein translation

Disease: Charcot-marie-tooth Disease, Axonal, Type 2w; Usher Syndrome, Type Iiib

NCBI Summary:Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P12081
NCBI GenInfo Identifier:135123
NCBI Gene ID:3035
NCBI Accession:P12081.2
UniProt Secondary Accession:P12081,B4DHQ1, B4DY73, D6REN6, J3KNE5,
UniProt Related Accession:P12081
Molecular Weight:54,847 Da
NCBI Full Name:Histidine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:histidyl-tRNA synthetase
NCBI Official Symbol:HARS��
NCBI Official Synonym Symbols:HRS; CMT2W; USH3B��
NCBI Protein Information:histidine--tRNA ligase, cytoplasmic
UniProt Protein Name:Histidine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Histidyl-tRNA synthetase; HisRS
Protein Family:Histidine--tRNA ligase
UniProt Gene Name:HARS��
UniProt Entry Name:SYHC_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P12081
Research Area:
Enzymes
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