Enzymes Recombinant Proteins
Human HAO1 Recombinant Protein (RPPB1794)
- SKU:
- RPPB1794
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UJM8
- Research Area:
- Enzymes
Description
| Product Name: | Human HAO1 Recombinant Protein |
| Product Code: | RPPB1794 |
| Size: | 10µg |
| Species: | Human |
| Target: | HAO1 |
| Synonyms: | Hydroxyacid oxidase 1, HAOX1, Glycolate oxidase, GOX, HAO1, GOX1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | HAO1 protein solution (1mg/ml) contains 20% glycerol, 20mM Tris-Hcl (pH8.0) and 0.5M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRMLRNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLVRQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIVAKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQGEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI |
| Biological Activity: | Specific activity is > 3000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C. |
Glycolate oxidase (HAO1) is a part of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. HAO1 catalyses the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate by reducing oxygen to hydrogen peroxide. HAO1 is expressed mainly in the liver and pancreas and is most active on twocarbon substrates such as glycolate. HAO1 isthe main cause of hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.
HAO1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-370 a.a) and having a molecular mass of 45.0kDa.HAO1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.1.3.15; Carbohydrate Metabolism - glyoxylate and dicarboxylate Chromosomal Location of Human Ortholog: 20p12 Cellular Component: peroxisomal matrix; peroxisome Molecular Function:glycolate oxidase activity; FMN binding; glyoxylate oxidase activity; (S)-2-hydroxy-acid oxidase activity; receptor binding Biological Process: glyoxylate metabolic process; response to oxidative stress; glycolate catabolic process; fatty acid alpha-oxidation |
| NCBI Summary: | This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UJM8 |
| NCBI GenInfo Identifier: | 13124294 |
| NCBI Gene ID: | 54363 |
| NCBI Accession: | Q9UJM8.1 |
| UniProt Secondary Accession: | Q9UJM8,Q14CQ0, Q9UPZ0, Q9Y3I7, |
| UniProt Related Accession: | Q9UJM8 |
| Molecular Weight: | 370 |
| NCBI Full Name: | Hydroxyacid oxidase 1 |
| NCBI Synonym Full Names: | hydroxyacid oxidase (glycolate oxidase) 1 |
| NCBI Official Symbol: | HAO1�� |
| NCBI Official Synonym Symbols: | GOX; GOX1; HAOX1�� |
| NCBI Protein Information: | hydroxyacid oxidase 1; glycolate oxidase; (S)-2-hydroxy-acid oxidase |
| UniProt Protein Name: | Hydroxyacid oxidase 1 |
| UniProt Synonym Protein Names: | Glycolate oxidase; GOX |
| Protein Family: | Hydroxyacid oxidase |
| UniProt Gene Name: | HAO1�� |
| UniProt Entry Name: | HAOX1_HUMAN |
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