Enzymes Recombinant Proteins
Human HAGH Recombinant Protein (RPPB1792)
- SKU:
- RPPB1792
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q16775
- Research Area:
- Enzymes
Description
| Product Name: | Human HAGH Recombinant Protein |
| Product Code: | RPPB1792 |
| Size: | 10µg |
| Species: | Human |
| Target: | HAGH |
| Synonyms: | GLX2, Glyoxalase II, GLO2, Hydroxyacyl Glutathione Hydrolase, HAGH1, GLXII, Hydroxyacylglutathione Hydrolase, hydroxyacylglutathione hydroxylase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The HAGH protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.5) and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD |
HAGH is a part of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. HAGH protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. HAGH appears only as a monomer and binds two zinc ions per subunit.
HAGH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-260a.a.) and having a molecular mass of 31.4kDa.HAGH is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 3.1.2.6; Carbohydrate Metabolism - pyruvate; Hydrolase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrial matrix; cytoplasm Molecular Function:hydroxyacylglutathione hydrolase activity; zinc ion binding Biological Process: glutathione biosynthetic process Disease: Hydroxyacyl Glutathione Hydrolase Deficiency |
| NCBI Summary: | The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] |
| UniProt Code: | Q16775 |
| NCBI GenInfo Identifier: | 257051015 |
| NCBI Gene ID: | 3029 |
| NCBI Accession: | Q16775.2 |
| UniProt Secondary Accession: | Q16775,A8K290, B4DP33, B4DRA7, E7EN93, |
| UniProt Related Accession: | Q16775 |
| Molecular Weight: | 308 |
| NCBI Full Name: | Hydroxyacylglutathione hydrolase, mitochondrial |
| NCBI Synonym Full Names: | hydroxyacylglutathione hydrolase |
| NCBI Official Symbol: | HAGH�� |
| NCBI Official Synonym Symbols: | GLO2; GLX2; GLXII; HAGH1�� |
| NCBI Protein Information: | hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase |
| UniProt Protein Name: | Hydroxyacylglutathione hydrolase, mitochondrial |
| UniProt Synonym Protein Names: | Glyoxalase II; Glx II |
| UniProt Gene Name: | HAGH�� |
| UniProt Entry Name: | GLO2_HUMAN |
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