Enzymes Recombinant Proteins

Human HAGH Recombinant Protein (RPPB1792)

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RPPB1792

Description

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Product Name:Human HAGH Recombinant Protein
Product Code:RPPB1792
Size:10µg
Species:Human
Target:HAGH
Synonyms:GLX2, Glyoxalase II, GLO2, Hydroxyacyl Glutathione Hydrolase, HAGH1, GLXII, Hydroxyacylglutathione Hydrolase, hydroxyacylglutathione hydroxylase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The HAGH protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.5) and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD

HAGH is a part of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. HAGH protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. HAGH appears only as a monomer and binds two zinc ions per subunit.

HAGH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-260a.a.) and having a molecular mass of 31.4kDa.HAGH is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; EC 3.1.2.6; Carbohydrate Metabolism - pyruvate; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function:hydroxyacylglutathione hydrolase activity; zinc ion binding

Biological Process: glutathione biosynthetic process

Disease: Hydroxyacyl Glutathione Hydrolase Deficiency

NCBI Summary:The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
UniProt Code:Q16775
NCBI GenInfo Identifier:257051015
NCBI Gene ID:3029
NCBI Accession:Q16775.2
UniProt Secondary Accession:Q16775,A8K290, B4DP33, B4DRA7, E7EN93,
UniProt Related Accession:Q16775
Molecular Weight:308
NCBI Full Name:Hydroxyacylglutathione hydrolase, mitochondrial
NCBI Synonym Full Names:hydroxyacylglutathione hydrolase
NCBI Official Symbol:HAGH��
NCBI Official Synonym Symbols:GLO2; GLX2; GLXII; HAGH1��
NCBI Protein Information:hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase
UniProt Protein Name:Hydroxyacylglutathione hydrolase, mitochondrial
UniProt Synonym Protein Names:Glyoxalase II; Glx II
UniProt Gene Name:HAGH��
UniProt Entry Name:GLO2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16775
Research Area:
Enzymes
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