Enzymes Recombinant Proteins

Human GZMB Recombinant Protein (RPPB1783)

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SKU:
RPPB1783
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P10144
Research Area:
Enzymes

Description

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Product Name:Human GZMB Recombinant Protein
Product Code:RPPB1783
Size:5µg
Species:Human
Target:GZMB
Synonyms:Granzyme B, T-Cell Serine Protease 1-3E, Cathepsin G-Like 1, Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cytotoxic T-Lymphocyte Proteinase 2, Cytotoxic Serine Protease B�, Human Lymphocyte Protein, Fragmentin 2, EC 3.4.21.79, CTSGL1, CTLA1�, CSPB�, CGL1, SECT�, C11, HLP�, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Lymphocyte Protease, Fragmentin-2�, Granzyme 2�, Granzyme-2�, EC 3.4.21, CTLA-1�, CGL-1, CSP-B, CCPI�, GRB. ���������
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GZMB protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) ,20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
Biological Activity:Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Granzyme-B (GZMB) is essential for target cell lysis in cell-mediated immune responses. GZMB is related to an activation cascade of caspases responsible for apoptosis execution. GZMB cuts caspase-3, -7, -9 and 10 to activate enzymes mediating apoptosis.

GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.
UniProt Protein Details:

Protein type:Apoptosis; EC 3.4.21.79; Protease

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytoplasm; cytosol; immunological synapse; intracellular membrane-bound organelle; membrane; nucleus

Molecular Function:protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: apoptosis; natural killer cell mediated cytotoxicity; protein processing

NCBI Summary:This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
UniProt Code:P10144
NCBI GenInfo Identifier:317373361
NCBI Gene ID:3002
NCBI Accession:P10144.2
UniProt Secondary Accession:P10144,Q8N1D2, Q9UCC1,
UniProt Related Accession:P10144
Molecular Weight:
NCBI Full Name:Granzyme B
NCBI Synonym Full Names:granzyme B
NCBI Official Symbol:GZMB��
NCBI Official Synonym Symbols:C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1��
NCBI Protein Information:granzyme B
UniProt Protein Name:Granzyme B
UniProt Synonym Protein Names:C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E
Protein Family:Granzyme
UniProt Gene Name:GZMB��
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