Enzymes Recombinant Proteins

Human GUK1 Recombinant Protein (RPPB2546)

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RPPB2546

Description

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Product Name:Human GUK1 Recombinant Protein
Product Code:RPPB2546
Size:10µg
Species:Human
Target:GUK1
Synonyms:GMK, GMP kinase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless clear solution.
Formulation:The GUK1 1mg/ml protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA
Biological Activity:Specific activity is > 100 units/mg and is defined asthe amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP perminute at pH 7.5 at 37C in coupled system with PK/LDH.

GUK1 is part of the guanylate kinase family. GUK1 occurs as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thus takes an important part in the recycling of GMP. Through its catalytic activity, GUK1 functions in regulation of the supply of guanine nucleotides to signal transduction pathways. GUK1 overexpression is related with pituitary adenocarcinomas, implicating that GUK1 is has a role in tumorigenesis.

GUK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (1-197 a.a.)and having a total molecular mass of 23.9 kDa. GUK1 is fused to a 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.

UniProt Protein Function:GUK1: Essential for recycling GMP and indirectly, cGMP. Belongs to the guanylate kinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.8

Chromosomal Location of Human Ortholog: 1q32-q41

Cellular Component: cytosol

Molecular Function:ATP binding; guanylate kinase activity

Biological Process: ATP metabolic process; dATP metabolic process; dGDP biosynthetic process; dGMP metabolic process; drug metabolic process; GDP biosynthetic process; GDP-mannose metabolic process; GMP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleotide phosphorylation; purine nucleotide metabolic process

NCBI Summary:The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
UniProt Code:Q16774
NCBI GenInfo Identifier:4504221
NCBI Gene ID:2987
NCBI Accession:NP_000849.1
UniProt Secondary Accession:Q16774,B1ANH1,
UniProt Related Accession:Q16774
Molecular Weight:37.6 kDa
NCBI Full Name:guanylate kinase isoform b
NCBI Synonym Full Names:guanylate kinase 1
NCBI Official Symbol:GUK1��
NCBI Official Synonym Symbols:GMK��
NCBI Protein Information:guanylate kinase
UniProt Protein Name:Guanylate kinase
UniProt Synonym Protein Names:GMP kinase
Protein Family:Guanylate kinase
UniProt Gene Name:GUK1��
UniProt Entry Name:KGUA_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16774
Research Area:
Enzymes
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