Human GTF2E2 Recombinant Protein (RPPB3647)
- SKU:
- RPPB3647
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P29084
Description
| Product Name: | Human GTF2E2 Recombinant Protein |
| Product Code: | RPPB3647 |
| Size: | 10µg |
| Species: | Human |
| Target: | GTF2E2 |
| Synonyms: | General transcription factor IIE polypeptide 2 beta 34kDa, transcription initiation factor IIE subunit beta, TFIIE-beta, TF2E2, TFIIE-B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GTF2E2 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK |
GTF2E2 is vital for transcription initiation by RNA polymerase II in conjunction with other general factors. General Transcription Factor IIE is a heterotetramer composed of two subunits of relatively high molecular mass 57,000 (GTF2E1) and two of 34,000 (GTF2E2). GTF2E2 is necessary in conjunction with GTF2E1 for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIE and TFIIH are essential for promoter clearance by RNA polymerase.
GTF2E2 Human Recombinant produced in E. coli is a single polypeptide chain containing 315 amino acids (1-291) and having a molecular mass of 35.6kDa.GTF2E2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GTF2E2: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE beta subunit family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 8p12 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| UniProt Code: | P29084 |
| NCBI GenInfo Identifier: | 135232 |
| NCBI Gene ID: | 2961 |
| NCBI Accession: | P29084.1 |
| UniProt Secondary Accession: | P29084,Q9H2B9, D3DSV2, |
| UniProt Related Accession: | P29084 |
| Molecular Weight: | 33,044 Da |
| NCBI Full Name: | Transcription initiation factor IIE subunit beta |
| NCBI Synonym Full Names: | general transcription factor IIE subunit 2 |
| NCBI Official Symbol: | GTF2E2�� |
| NCBI Official Synonym Symbols: | FE; TTD6; TF2E2; TFIIE-B�� |
| NCBI Protein Information: | transcription initiation factor IIE subunit beta |
| UniProt Protein Name: | Transcription initiation factor IIE subunit beta |
| UniProt Synonym Protein Names: | General transcription factor IIE subunit 2 |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2E2�� |
| UniProt Entry Name: | T2EB_HUMAN |
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