Human GTF2B Recombinant Protein (RPPB3646)
- SKU:
- RPPB3646
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q00403
Description
| Product Name: | Human GTF2B Recombinant Protein |
| Product Code: | RPPB3646 |
| Size: | 25µg |
| Species: | Human |
| Target: | GTF2B |
| Synonyms: | TF2B, TFIIB, GTF2B, Transcription initiation factor IIB, General transcription factor TFIIB, S300-II. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GTF2B solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL |
GTF2B is one of the ubiquitous factors needed for transcription initiation by RNA polymerase II preinitiation factor. GTF2B localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. GTF2 plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. GTF2B is involved in the selection of the transcription start site.
GTF2B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 336 amino acids (1-316 a.a.) and having a molecular mass of 36.9 kDa. The GTF2B is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GTF2B: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Associates with TFIID-IIA (DA complex) to form TFIID-IIA- IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1. Interacts with Epstein-Barr virus EBNA2. Belongs to the TFIIB family. |
| UniProt Protein Details: | Protein type:Acetyltransferase; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 1p22-p21 Cellular Component: nucleolus; nucleoplasm; nucleus Molecular Function:protein binding; thyroid hormone receptor binding; transcription factor binding Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q00403 |
| NCBI GenInfo Identifier: | 135629 |
| NCBI Gene ID: | 2959 |
| NCBI Accession: | Q00403.1 |
| UniProt Secondary Accession: | Q00403,Q5JS30, A8K1A7, |
| UniProt Related Accession: | Q00403 |
| Molecular Weight: | 34,833 Da |
| NCBI Full Name: | Transcription initiation factor IIB |
| NCBI Synonym Full Names: | general transcription factor IIB |
| NCBI Official Symbol: | GTF2B�� |
| NCBI Official Synonym Symbols: | TF2B; TFIIB�� |
| NCBI Protein Information: | transcription initiation factor IIB |
| UniProt Protein Name: | Transcription initiation factor IIB |
| UniProt Synonym Protein Names: | General transcription factor TFIIB; S300-II |
| Protein Family: | Transcription initiation factor |
| UniProt Gene Name: | GTF2B�� |
| UniProt Entry Name: | TF2B_HUMAN |
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