Enzymes Recombinant Proteins
Human GSTM5 Recombinant Protein (RPPB1769)
- SKU:
- RPPB1769
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P46439
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTM5 Recombinant Protein |
| Product Code: | RPPB1769 |
| Size: | 10µg |
| Species: | Human |
| Target: | GSTM5 |
| Synonyms: | Glutathione S-transferase mu 5, GSTM5-5, GST class-mu 5, GTM5, glutathione S-alkyltransferase M5, S-(hydroxyalkyl) glutathione lyase M5, EC 2.5.1.18. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GSTM5 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQWLNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCYDPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFISRFEGLKKISA YMKSSQFLRG LLFGKSATWN SK |
| Biological Activity: | Specific activity is > 90,000 pmol/min/ug, and isdefined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4- dinitrobenzene(CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
Glutathione S-transferase mu 5 (GSTM5) belongs to the glutathione s-transferase (GST) family of proteins. There are 8 families of GST proteins, specifically alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is comprised of proteins which have various functions throughout the cell. GSTM5 belongs to the mu class of enzymes which function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. GSTM5 has an imperative role in detoxification. GSTM5 conjugates reduced glutathione to a large number of exogenous and endogenous hydrophobic electrophiles.
GSTM5 Human Recombinant produced in E. coli is a single polypeptide chain containing 242 amino acids (1-218) and having a molecular mass of 28.2 kDa.GSTM5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTM5: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytosol Molecular Function:glutathione transferase activity; protein binding Biological Process: glutathione metabolic process |
| NCBI Summary: | Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46439 |
| NCBI GenInfo Identifier: | 67476963 |
| NCBI Gene ID: | 2949 |
| NCBI Accession: | P46439.3 |
| UniProt Secondary Accession: | P46439,Q6PD78, A8K0V8, |
| UniProt Related Accession: | P46439 |
| Molecular Weight: | 25,675 Da |
| NCBI Full Name: | Glutathione S-transferase Mu 5 |
| NCBI Synonym Full Names: | glutathione S-transferase mu 5 |
| NCBI Official Symbol: | GSTM5�� |
| NCBI Official Synonym Symbols: | GTM5; GSTM5-5�� |
| NCBI Protein Information: | glutathione S-transferase Mu 5 |
| UniProt Protein Name: | Glutathione S-transferase Mu 5 |
| UniProt Synonym Protein Names: | GST class-mu 5; GSTM5-5 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTM5�� |
| UniProt Entry Name: | GSTM5_HUMAN |
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