Human Growth Factor Array (41 targets) (SARB0045)

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SKU:
SARB0045

Description

system_update_altDatasheet
Product Sku:SARB0045
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:41
Gene Symbols:AREG, CSF1, CSF1R, CSF2, CSF3, EGF, EGFR, FGF2, FGF4, FGF6|FGF7, FIGF, FLT4, GDNF, HBEGF, HGF, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, KDR, KIT, KITLG, NGF, NTF3, NTF4, PDGFA, PDGFB, PDGFRA, PDGFRB, PGF, TGFA, TGFB1, TGFB2, TGFB3, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Amphiregulin
  • Gene Symbol: AREG
  • Gene ID: 374
  • Accession #: P15514
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 2247
  • Accession #: P09038
beta-NGF
  • Gene Symbol: NGF
  • Gene ID: 4803
  • Accession #: P01138
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
EGFR
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
FGF-4
  • Gene Symbol: FGF4
  • Gene ID: 2249
  • Accession #: P08620
FGF-6
  • Gene Symbol: FGF6
  • Gene ID: 2251
  • Accession #: P10767
FGF-7 (KGF)
  • Gene Symbol: FGF7
  • Gene ID: 2252
  • Accession #: P21781
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
HB-EGF
  • Gene Symbol: HBEGF
  • Gene ID: 1839
  • Accession #: Q99075
HGF
  • Gene Symbol: HGF
  • Gene ID: 3082
  • Accession #: P14210
IGFBP-1
  • Gene Symbol: IGFBP1
  • Gene ID: 3484
  • Accession #: P08833
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: 3485
  • Accession #: P18065
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: 3486
  • Accession #: P17936
IGFBP-4
  • Gene Symbol: IGFBP4
  • Gene ID: 3487
  • Accession #: P22692
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: 3489
  • Accession #: P24592
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
IGF-1 R
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
M-CSF R
  • Gene Symbol: CSF1R
  • Gene ID: 1436
  • Accession #: P07333
NT-3
  • Gene Symbol: NTF3
  • Gene ID: 4908
  • Accession #: P20783
NT-4
  • Gene Symbol: NTF4
  • Gene ID: 4909
  • Accession #: P34130
PDGF R alpha
  • Gene Symbol: PDGFRA
  • Gene ID: 5156
  • Accession #: P16234
PDGF R beta
  • Gene Symbol: PDGFRB
  • Gene ID: 5159
  • Accession #: P09619
PDGF-AA
  • Gene Symbol: PDGFA
  • Gene ID: 5154
  • Accession #: P04085
PDGF-AB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
SCF
  • Gene Symbol: KITLG
  • Gene ID: 4254
  • Accession #: P21583
SCF R (CD117/c-kit)
  • Gene Symbol: KIT
  • Gene ID: 3815
  • Accession #: P10721
TGF alpha
  • Gene Symbol: TGFA
  • Gene ID: 7039
  • Accession #: P01135
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: 3791
  • Accession #: P35968
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: 2324
  • Accession #: P35916
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: 2277
  • Accession #: O43915
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Amphiregulin
Analytes:
bFGF
Analytes:
beta-NGF
Analytes:
EGF
Analytes:
EGFR
Analytes:
FGF-4
Analytes:
FGF-6
Analytes:
FGF-7 (KGF)
Analytes:
GCSF
Analytes:
GDNF
Analytes:
GM-CSF
Analytes:
HB-EGF
Analytes:
HGF
Analytes:
IGFBP-1
Analytes:
IGFBP-2
Analytes:
IGFBP-3
Analytes:
IGFBP-4
Analytes:
IGFBP-6
Analytes:
IGF-1
Analytes:
IGF-1 R
Analytes:
IGF-2
Analytes:
M-CSF
Analytes:
M-CSF R
Analytes:
NT-3
Analytes:
NT-4
Analytes:
PDGF R alpha
Analytes:
PDGF R beta
Analytes:
PDGF-AA
Analytes:
PDGF-AB
Analytes:
PDGF-BB
Analytes:
PLGF
Analytes:
SCF
Analytes:
SCF R (CD117/c-kit)
Analytes:
TGF alpha
Analytes:
TGF beta 1
Analytes:
TGF beta 2
Analytes:
TGF beta 3
Analytes:
VEGF-A
Analytes:
VEGFR2
Analytes:
VEGFR3
Analytes:
VEGF-D
Reactivity:
Human
Applications:
Multiplex Array
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