Enzymes Recombinant Proteins

Human GPX1 Recombinant Protein (RPPB1743)

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SKU:
RPPB1743
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P07203
Research Area:
Enzymes

Description

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Product Name:Human GPX1 Recombinant Protein
Product Code:RPPB1743
Size:20µg
Species:Human
Target:GPX1
Synonyms:Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1, GPXD, GSHPX1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The GPX1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 30% glycerol and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MCAARLAAAA AAAQSVYAFS ARPLAGGEPV SLGSLRGKVL LIENVASLCG TTVRDYTQMN ELQRRLGPRG LVVLGFPCNQ FGHQENAKNE EILNSLKYVR PGGGFEPNFM LFEKCEVNGA GAHPLFAFLR EALPAPSDDA TALMTDPKLI TWSPVCRNDV AWNFEKFLVG PDGVPLRRYS RRFQTIDIEP DIEALLSQGP SCA

Glutathione peroxidase 1 (GPX1) is a member of the glutathione peroxidase family, consisting of 8 identified glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase serves in the detoxification of hydrogen peroxide, and is one of the most vital antioxidant enzymes in humans. The GPX1 is a component of the enzymatic antioxidant defense, preventing oxidative damage to DNA, proteins and lipids by detoxifying hydrogen and lipid peroxides which may contribute to prostate cancer development. GPX1 is one of only a small number of proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Furthermore, the GPX1 protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes 3 alleles with 5, 6 or 7 alanine (ALA) repeats in this sequence. The allele with 5 ALA repeats is significantly linked to breast cancer risk.

GPX1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-203) and having a molecular mass of 24.2kDa.GPX1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Protects the hemoglobin in erythrocytes from oxidative breakdown.
NCBI Summary:The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21. [provided by RefSeq, Aug 2017]
UniProt Code:P07203
NCBI GenInfo Identifier:311033481
NCBI Gene ID:2876
NCBI Accession:P07203.4
UniProt Secondary Accession:P07203,Q7Z5H1, Q9BW12, E9PAS1,
UniProt Related Accession:P07203
Molecular Weight:10,289 Da
NCBI Full Name:Glutathione peroxidase 1
NCBI Synonym Full Names:glutathione peroxidase 1
NCBI Official Symbol:GPX1��
NCBI Official Synonym Symbols:GPXD; GSHPX1��
NCBI Protein Information:glutathione peroxidase 1
UniProt Protein Name:Glutathione peroxidase 1
UniProt Synonym Protein Names:Cellular glutathione peroxidase
Protein Family:Hydroperoxy fatty acid reductase
UniProt Gene Name:GPX1��
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