Enzymes Recombinant Proteins

Human GPT Recombinant Protein (RPPB1736)

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RPPB1736

Description

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Product Name:Human GPT Recombinant Protein
Product Code:RPPB1736
Size:10µg
Species:Human
Target:GPT
Synonyms:Glutamic-pyruvate transaminase (alanineaminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC2.6.1.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GPT solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Biological Activity:Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.

GPT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-496) and having a molecular mass of 56.8 kDa.GPT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
UniProt Protein Details:

Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function:alanine transaminase activity

Biological Process: amino acid biosynthetic process

NCBI Summary:This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
UniProt Code:P24298
NCBI GenInfo Identifier:46577683
NCBI Gene ID:2875
NCBI Accession:P24298.3
UniProt Secondary Accession:P24298,P78398, Q93076, B0YJ18, D3DWM7,
UniProt Related Accession:P24298
Molecular Weight:54,637 Da
NCBI Full Name:Alanine aminotransferase 1
NCBI Synonym Full Names:glutamic--pyruvic transaminase
NCBI Official Symbol:GPT��
NCBI Official Synonym Symbols:AAT1; ALT1; GPT1��
NCBI Protein Information:alanine aminotransferase 1
UniProt Protein Name:Alanine aminotransferase 1
UniProt Synonym Protein Names:Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family:Alanine aminotransferase
UniProt Gene Name:GPT��
UniProt Entry Name:ALAT1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P24298
Research Area:
Enzymes
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