Enzymes Recombinant Proteins
Human GPT Recombinant Protein (RPPB1736)
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- SKU:
- RPPB1736
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P24298
- Research Area:
- Enzymes
Description
| Product Name: | Human GPT Recombinant Protein |
| Product Code: | RPPB1736 |
| Size: | 10µg |
| Species: | Human |
| Target: | GPT |
| Synonyms: | Glutamic-pyruvate transaminase (alanineaminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC2.6.1.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GPT solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS |
| Biological Activity: | Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C. |
GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.
GPT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-496) and having a molecular mass of 56.8 kDa.GPT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. |
| UniProt Protein Details: | Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:alanine transaminase activity Biological Process: amino acid biosynthetic process |
| NCBI Summary: | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| UniProt Code: | P24298 |
| NCBI GenInfo Identifier: | 46577683 |
| NCBI Gene ID: | 2875 |
| NCBI Accession: | P24298.3 |
| UniProt Secondary Accession: | P24298,P78398, Q93076, B0YJ18, D3DWM7, |
| UniProt Related Accession: | P24298 |
| Molecular Weight: | 54,637 Da |
| NCBI Full Name: | Alanine aminotransferase 1 |
| NCBI Synonym Full Names: | glutamic--pyruvic transaminase |
| NCBI Official Symbol: | GPT�� |
| NCBI Official Synonym Symbols: | AAT1; ALT1; GPT1�� |
| NCBI Protein Information: | alanine aminotransferase 1 |
| UniProt Protein Name: | Alanine aminotransferase 1 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1 |
| Protein Family: | Alanine aminotransferase |
| UniProt Gene Name: | GPT�� |
| UniProt Entry Name: | ALAT1_HUMAN |
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