Enzymes Recombinant Proteins

Human GOT2 Recombinant Protein (RPPB1726)

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SKU:
RPPB1726
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00505
Research Area:
Enzymes

Description

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Product Name:Human GOT2 Recombinant Protein
Product Code:RPPB1726
Size:10µg
Species:Human
Target:GOT2
Synonyms:EC 2.6.1.1, Aspartate aminotransferase 1, Transaminase A, GIG18.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The GOT2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYVLPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQRFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKRNLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVESQLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSSNVGYLAHAIH QVTK
Biological Activity:Specific activity is > 60 units/mg, and is defined asthe amount of enzyme that convert 1umole of ?-ketoglutarate to L-Glutamate per minuteat pH 8.0 at 25C.

GOT2 is a pyridoxal phosphate-dependent enzyme which is found in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2. GOT2 is invloved in amino acid metabolism and the urea and tricarboxylic acid cycles. The 2 enzymes are homodimeric and demonstrate close homology.

GOT2 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (30-430 a.a.) and having a molecular mass of 47kDa. The GOT2 fused to a 23 amino acid his tag at N-terminus and is purified by proprietary chromatographic techniques.

UniProt Code:P00505
NCBI GenInfo Identifier:73486658
NCBI Gene ID:2806
NCBI Accession:NP_002071.2
UniProt Secondary Accession:P00505,Q53FL3, Q9BWA3,
UniProt Related Accession:P00505
Molecular Weight:430
NCBI Full Name:aspartate aminotransferase, mitochondrial
NCBI Synonym Full Names:glutamic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol:GOT2��
NCBI Official Synonym Symbols:KAT4; KATIV; mitAAT��
NCBI Protein Information:aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate tr
UniProt Protein Name:Aspartate aminotransferase, mitochondrial
UniProt Synonym Protein Names:Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A
UniProt Gene Name:GOT2��
UniProt Entry Name:AATM_HUMAN
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