Enzymes Recombinant Proteins

Human GOT1 Recombinant Protein (RPPB1723)

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SKU:
RPPB1723
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17174
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human GOT1 Recombinant Protein
Product Code:RPPB1723
Size:10µg
Species:Human
Target:GOT1
Synonyms:EC 2.6.1.1, Aspartate aminotransferase 1, Transaminase A, GIG18.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The GOT1 solution (0.5mg/ml) containing 20mM Tris-HCl pH-8.0, 2mM DTT, 0.1M NaCl & 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
Biological Activity:Specific activity is > 50 units/mg, and is defined asthe amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate perminute at pH 8.0 at 25C.

GOT1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, which participate in amino acid metabolism and the urea and tricarboxylic acid cycles. Both enzymes are homodimeric and show close homology.

GOT1 Human Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 433 amino acids (1-413 a.a.) and having a molecular mass of 48.4 kDa. The GOT1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Transferase; EC 2.6.1.1; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.3; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: lysosome; cytoplasm; nerve terminal; cytosol; nucleus

Molecular Function:cysteine transaminase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; aspartate biosynthetic process; glucose metabolic process; pathogenesis; 2-oxoglutarate metabolic process; gluconeogenesis; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1

NCBI Summary:Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
UniProt Code:P17174
NCBI GenInfo Identifier:5902703
NCBI Gene ID:2805
NCBI Accession:P17174.3
UniProt Secondary Accession:P17174,Q5VW80, B2R6R7, B7Z7E9,
UniProt Related Accession:P17174
Molecular Weight:Calculated MW: 44kDa/46kDaObserved MW: 46kDa
NCBI Full Name:Aspartate aminotransferase, cytoplasmic
NCBI Synonym Full Names:glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol:GOT1��
NCBI Official Synonym Symbols:cCAT; GIG18; cAspAT; ASTQTL1��
NCBI Protein Information:aspartate aminotransferase, cytoplasmic; transaminase A; aspartate transaminase 1; aspartate aminotransferase 1; growth-inhibiting protein 18; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
UniProt Protein Name:Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names:Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family:Protein transport protein
UniProt Gene Name:GOT1��
UniProt Entry Name:AATC_HUMAN
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Metabolism Recombinant Proteins

system_update_altDatasheetHuman GOT1 Recombinant Protein Glutamate Oxaloacetate Transaminase 1 (GOT1) is a cytoplasmic protein. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotran