Human GOPC Recombinant Protein (RPPB3628)

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SKU:
RPPB3628
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HD26

Description

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Product Name:Human GOPC Recombinant Protein
Product Code:RPPB3628
Size:25µg
Species:Human
Target:GOPC
Synonyms:CAL, dJ94G16.2, FIG, GOPC1, PIST, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma, GOPC.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GOPC protein solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQQRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDELEGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY

GOPC is a PDZ domain-containing Golgi protein which binds the extreme C terminus of proteins in a sequence-specific manner. GOPC participates in intracellular protein trafficking and degradation. GOPC regulates CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. GOPC regulates the intracellular trafficking of the ADR1B receptor. GOPC takes part in autophagy. GOPC over expression results in CFTR intracellular retention and degradation in the lysosomes.

GOPC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (278-454 a.a.) and having a molecular mass of 21.5 kDa. The GOPC is fused to a 20 amino acid His Tag and purified by conventional chromatography.

UniProt Protein Function:PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: Golgi membrane; Golgi apparatus; postsynaptic membrane; trans-Golgi network transport vesicle; protein complex; membrane; postsynaptic density; cytoplasm; dendrite; plasma membrane; cell junction

Molecular Function:protein C-terminus binding; protein binding; protein homodimerization activity; frizzled binding

Biological Process: Golgi to plasma membrane transport; protein transport; ER to Golgi vesicle-mediated transport; apical protein localization; regulation of catalytic activity; spermatid nuclear differentiation; protein homooligomerization; cytoplasmic sequestering of CFTR protein

Disease: Spermatogenic Failure 6

NCBI Summary:This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:Q9HD26
NCBI GenInfo Identifier:74762751
NCBI Gene ID:57120
NCBI Accession:Q9HD26.1
UniProt Secondary Accession:Q9HD26,Q59FS4, Q969U8, A6NM30,
UniProt Related Accession:Q9HD26
Molecular Weight:462
NCBI Full Name:Golgi-associated PDZ and coiled-coil motif-containing protein
NCBI Synonym Full Names:golgi-associated PDZ and coiled-coil motif containing
NCBI Official Symbol:GOPC��
NCBI Official Synonym Symbols:CAL; FIG; PIST; GOPC1; dJ94G16.2��
NCBI Protein Information:Golgi-associated PDZ and coiled-coil motif-containing protein; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated PDZ and coiled-coil motif containing protein; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
UniProt Protein Name:Golgi-associated PDZ and coiled-coil motif-containing protein
UniProt Synonym Protein Names:CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST
Protein Family:Golgi-associated PDZ and coiled-coil motif-containing protein
UniProt Gene Name:GOPC��
UniProt Entry Name:GOPC_HUMAN
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