Enzymes Recombinant Proteins

Human GMPS Recombinant Protein (RPPB1716)

(No reviews yet) Write a Review
SKU:
RPPB1716

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human GMPS Recombinant Protein
Product Code:RPPB1716
Size:10µg
Species:Human
Target:GMPS
Synonyms:GMP synthase [glutamine-hydrolyzing], GMP synthetase, Glutamine amidotransferase, GMPS, GMP synthase, guanosine 5'-monophosphate synthase, MLL/GMPS fusion protein.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GMPS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMALCNG DSKLENAGGD LKDGHHHYEG AVVILDAGAQ YGKVIDRRVR ELFVQSEIFP LETPAFAIKE QGFRAIIISG GPNSVYAEDA PWFDPAIFTI GKPVLGICYG MQMMNKVFGG TVHKKSVRED GVFNISVDNT CSLFRGLQKE EVVLLTHGDS VDKVADGFKV VARSGNIVAG IANESKKLYG AQFHPEVGLT ENGKVILKNF LYDIAGCSGT FTVQNRELEC IREIKERVGT SKVLVLLSGG VDSTVCTALL NRALNQEQVI AVHIDNGFMR KRESQSVEEA LKKLGIQVKV INAAHSFYNG TTTLPISDED RTPRKRISKT LNMTTSPEEK RKIIGDTFVK IANEVIGEMN LKPEEVFLAQ GTLRPDLIES ASLVASGKAE LIKTHHNDTE LIRKLREEGK VIEPLKDFHK DEVRILGREL GLPEELVSRH PFPGPGLAIR VICAEEPYIC KDFPETNNIL KIVADFSASV KKPHTLLQRV KACTTEEDQE KLMQITSLHS LNAFLLPIKT VGVQGDCRSY SYVCGISSKD EPDWESLIFL ARLIPRMCHN VNRVVYIFGP PVKEPPTDVT PTFLTTGVLS TLRQADFEAH NILRESGYAG KISQMPVILT PLHFDRDPLQ KQPSCQRSVV IRTFITSDFM TGIPATPGNE IPVEVVLKMV TEIKKIPGIS RIMYDLTSKP PGTTEWE

GMP synthase (GMPS) is involved in purine biosynthesis. GMPS, which is a homodimer, catalyzes the last step in the GMP synthesis pathway, specifically the ATP-dependent amination of XMP to GMP. GMPS is comprised of one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it communicates its catalytic activity. GMPS is engaged in the de novo synthesis of nucleotides which are not only vital for DNA and RNA synthesis, but also supply GTP, which is involved in sevral cellular processes important for cell division. GMPS gene chromosomal translocations are linked with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis.

GMPS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 717 amino acids (1-693) and having a molecular mass of 79.2kDa.GMPS is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
UniProt Protein Details:

Protein type:Oncoprotein; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Ligase; EC 6.3.5.2

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: cytoplasm; cytosol

Molecular Function:GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity

Biological Process: purine base biosynthetic process; purine ribonucleoside monophosphate biosynthetic process

Disease: Leukemia, Acute Myeloid

NCBI Summary:In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]
UniProt Code:P49915
NCBI GenInfo Identifier:1708072
NCBI Gene ID:8833
NCBI Accession:P49915.1
UniProt Secondary Accession:P49915,A8K639, B4DXV7, F8W720,
UniProt Related Accession:P49915
Molecular Weight:65,929 Da
NCBI Full Name:GMP synthase
NCBI Synonym Full Names:guanine monophosphate synthase
NCBI Official Symbol:GMPS��
NCBI Protein Information:GMP synthase [glutamine-hydrolyzing]
UniProt Protein Name:GMP synthase [glutamine-hydrolyzing]
UniProt Synonym Protein Names:GMP synthetase; Glutamine amidotransferase
UniProt Gene Name:GMPS��
UniProt Entry Name:GUAA_HUMAN
View AllClose

Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49915
Research Area:
Enzymes
View AllClose

Related Products