Human GMNN Recombinant Protein (RPPB3612)
- SKU:
- RPPB3612
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O75496
Description
| Product Name: | Human GMNN Recombinant Protein |
| Product Code: | RPPB3612 |
| Size: | 20µg |
| Species: | Human |
| Target: | GMNN |
| Synonyms: | GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The protein solution contains 20mM Tris pH 8, 100mM NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI |
Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the �S phase� of the cell cycle. Inhibition of Geminin during the �S phase� (by RNAi) results in an additional round of replication of portions of the genome. During the �M phase� of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. Geminin thus guarantees that only one round of replication occurs during each cell cycle. It was discovered that Geminin is overexpressed in a number of malignancies and cancer cell lines. This maintains the concept that Geminin has also a positive role in DNA replication and cell cycle progression. Geminin accumulates through S, G2 and M phases of the cell cycle but is absent during the G1 phase. During the metaphase/anaphase transition of mitosis Geminin levels decrease.
Geminin Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 245 amino acids and having a molecular mass of 27.7 kDa.
| UniProt Protein Function: | Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus Molecular Function:protein binding; histone deacetylase binding; transcription corepressor activity Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle |
| NCBI Summary: | This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O75496 |
| NCBI GenInfo Identifier: | 20454908 |
| NCBI Gene ID: | 51053 |
| NCBI Accession: | O75496.1 |
| UniProt Secondary Accession: | O75496,Q9H1Z1, B3KMM8, |
| UniProt Related Accession: | O75496 |
| Molecular Weight: | 209 |
| NCBI Full Name: | Geminin |
| NCBI Synonym Full Names: | geminin, DNA replication inhibitor |
| NCBI Official Symbol: | GMNN�� |
| NCBI Official Synonym Symbols: | Gem�� |
| NCBI Protein Information: | geminin |
| UniProt Protein Name: | Geminin |
| Protein Family: | Geminin |
| UniProt Gene Name: | GMNN�� |
| UniProt Entry Name: | GEMI_HUMAN |
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