Growth Factors & Cytokines Recombinant Proteins
Human GM CSF Recombinant Protein (RPPB0355)
- SKU:
- RPPB0355
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P04141
- Research Area:
- Growth Factors & Cytokines
Description
Product Name: | Human GM CSF Recombinant Protein |
Product Code: | RPPB0355 |
Size: | 10µg |
Species: | Human |
Target: | GM CSF |
Synonyms: | CSF-2, MGI-1GM, GMCSF, Pluripoietin-alpha, Molgramostin, Sargramostim. |
Source: | Insect Cells |
Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation: | The protein was lyophilized with PBS. |
Solubility: | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
Stability: | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Purity: | Greater than 98.0% as determined by SDS-PAGE. |
Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser |
Biological Activity: | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is�< 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg. |
GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
GM-CSF Human Recombinant produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids (18-144) and having a molecular mass of 14.6kDa. GM-CSF is fused to a C-terminal His -tag (6x His) and purified by proprietary chromatographic techniques.
UniProt Protein Function: | GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family. |
UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cytokine Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular space; extracellular region Molecular Function:protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication |
NCBI Summary: | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
UniProt Code: | P04141 |
NCBI GenInfo Identifier: | 117561 |
NCBI Gene ID: | 1437 |
NCBI Accession: | P04141.1 |
UniProt Secondary Accession: | P04141,Q14CE8, Q2VPI8, Q8NFI6, |
UniProt Related Accession: | P04141 |
Molecular Weight: | 16,295 Da |
NCBI Full Name: | Granulocyte-macrophage colony-stimulating factor |
NCBI Synonym Full Names: | colony stimulating factor 2 (granulocyte-macrophage) |
NCBI Official Symbol: | CSF2�� |
NCBI Official Synonym Symbols: | GMCSF�� |
NCBI Protein Information: | granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim |
UniProt Protein Name: | Granulocyte-macrophage colony-stimulating factor |
UniProt Synonym Protein Names: | Colony-stimulating factor; CSF; Molgramostin; Sargramostim |
Protein Family: | Granulocyte-macrophage colony-stimulating factor |
UniProt Gene Name: | CSF2�� |
UniProt Entry Name: | CSF2_HUMAN |