Description
| Product Name: | Human GLTPD1 Recombinant Protein |
| Product Code: | RPPB3402 |
| Size: | 10µg |
| Species: | Human |
| Target: | GLTPD1 |
| Synonyms: | Ceramide-1-Phosphate Transfer Protein, GlycolipidTransfer Protein Domain-Containing Protein 1, Glycolipid Transfer ProteinDomain Containing 1, GLTP Domain-Containing Protein 1, GLTPD1, GLTPD1, Ceramide-1-phosphatetransfer protein, CPTP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GLTPD1 protein solution (0.25mg/ml) containing Phosphatebuffered saline (pH7.4), 50% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDDSETG FNLKVVLVSF KQCLDEKEEV LLDPYIASWK GLVRFLNSLGTIFSFISKDV VSKLRIMERL RGGPQSEHYR SLQAMVAHEL SNRLVDLERR SHHPESGCRT VLRLHRALHWLQLFLEGLRT SPEDARTSAL CADSYNASLA AYHPWVVRRAVTVAFCTLPT REVFLEAMNV GPPEQAVQML GEALPFIQRV YNVSQKLYAEHSLLDLP |
Glycolipid Transfer Protein Domain Containing 1 (GLTPD1) belongs to theGLTP family. GLTPD1 mediates the intracellular transfer of ceramide-1-phosphatebetween organelle membranes and the cell membrane. GLTPD1 is essential fornormal structure of the Golgi stacks. In addition, GLTPD1 is able to bindphosphoceramides with a diversity of aliphatic chains, however GLTPD1 has apreference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains.Moreover, GLTPD1 is inefficient with phosphoceramides which contains lignoceryl(C24:0).� GLTPD1 participates in the regulation of the cellular levels ofceramide-1-phosphate, and in that way contributes to the regulation ofphospholipase PLA2G4A activity and the release of arachidonic acid.
GLTPD1 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 237 amino acids (1-214 a.a) andhaving a molecular mass of 26.8kDa.�GLTPD1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Mediates the intracellular transfer of ceramide-1-phosphate (C1P) between organelle membranes and the cell membrane. Required for normal structure of the Golgi stacks. Can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). Plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Has no activity with galactosylceramide, lactosylceramide, sphingomyelin, phosphatidylcholine, phosphatidic acid and ceramide. C1P transfer is stimulated by phosphatidylserine in C1P source vesicles (PubMed:28011644). Regulates autophagy, inflammasome mediated IL1B and IL18 processing, and pyroptosis, but not apoptosis (PubMed:29164996). |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q5TA50 |
| NCBI GenInfo Identifier: | 71274150 |
| NCBI Gene ID: | 80772 |
| NCBI Accession: | NP_001025056.1 |
| UniProt Secondary Accession: | Q5TA50,Q4G0E6, Q7L5A4 |
| UniProt Related Accession: | Q5TA50 |
| Molecular Weight: | 24,365 Da |
| NCBI Full Name: | ceramide-1-phosphate transfer protein |
| NCBI Synonym Full Names: | ceramide-1-phosphate transfer protein |
| NCBI Official Symbol: | CPTP�� |
| NCBI Official Synonym Symbols: | GLTPD1�� |
| NCBI Protein Information: | ceramide-1-phosphate transfer protein |
| UniProt Protein Name: | Ceramide-1-phosphate transfer protein |
| UniProt Synonym Protein Names: | Glycolipid transfer protein domain-containing protein 1Curated; GLTP domain-containing protein 11 PublicationManual assertion based on opinion iniRef.8"Non-vesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids."Simanshu D.K., Kamlekar R.K., Wijesinghe D.S., Zou X., Zhai X., Mishra S.K., Molotkovsky J.G., Malinina L., Hinchcliffe E.H., Chalfant C.E., Brown R.E., Patel D.J.Nature 500:463-467(2013) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH CERAMIDE-1-PHOSPHATE, FUNCTION, SUBCELLULAR LOCATION, LIPID-BINDING, MUTAGENESIS OF PHE-42; LEU-43; PHE-50; ILE-53; ASP-56; LYS-60; ARG-97; ARG-106; ARG-110; ARG-113; TRP-117; LEU-118; TYR-149 AND VAL-158, TISSUE SPECIFICITY. |
| Protein Family: | Ceramide-1-phosphate transfer protein |
| UniProt Gene Name: | CPTP�� |
| UniProt Entry Name: |
