Human GINS4 Recombinant Protein (RPPB3594)

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RPPB3594

Description

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Product Name:Human GINS4 Recombinant Protein
Product Code:RPPB3594
Size:10µg
Species:Human
Target:GINS4
Synonyms:SLD5, GINS complex subunit 4, DNA replication complex GINS protein SLD5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:GINS4 protein solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl,20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK GSQHLIRYKT IAPLVASGAV QLI

GINS4, a member of the GINS4/SLD5 family, has a key part in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is significant for GINS complex assembly. GINS complex connects favorably to singlestranded DNA. Recombinant human GINS4 protein, fused to His-tag at N-terminus was purified by using conventional chromatography techniques.

GINS4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 243 amino acids (1-223 a.a.) and having a molecular mass of 28.2kDa.GINS4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GINS4: The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. Belongs to the GINS4/SLD5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: nucleoplasm; cytoplasm

Molecular Function:protein binding

Biological Process: mitotic cell cycle; DNA strand elongation during DNA replication; inner cell mass cell proliferation

NCBI Summary:The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM, Mar 2008]
UniProt Code:Q9BRT9
NCBI GenInfo Identifier:14150122
NCBI Gene ID:84296
NCBI Accession:NP_115712.1
UniProt Secondary Accession:Q9BRT9,Q99LZ3, Q499W2,
UniProt Related Accession:Q9BRT9
Molecular Weight:11,844 Da
NCBI Full Name:DNA replication complex GINS protein SLD5
NCBI Synonym Full Names:GINS complex subunit 4 (Sld5 homolog)
NCBI Official Symbol:GINS4��
NCBI Official Synonym Symbols:SLD5��
NCBI Protein Information:DNA replication complex GINS protein SLD5; SLD5 homolog
UniProt Protein Name:DNA replication complex GINS protein SLD5
UniProt Synonym Protein Names:GINS complex subunit 4DNA replication complex GINS protein SLD5, N-terminally processed
Protein Family:DNA replication complex GINS protein
UniProt Gene Name:GINS4��
UniProt Entry Name:SLD5_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BRT9
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