Neurotrophins Recombinant Proteins

Human GFRA1 Recombinant Protein (RPPB0298)

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SKU:
RPPB0298
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P56159
Research Area:
Neurotrophins

Description

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Product Name:Human GFRA1 Recombinant Protein
Product Code:RPPB0298
Size:10µg
Species:Human
Target:GFRA1
Synonyms:GDNF receptor alpha-1, GDNFR-alpha-1, GFRalpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, GDNFRA1, RET1L2, RETL1, Glial Cell LineDerived Neurotrophic Factor Receptor Alpha, TRNR1, GPILinked Anchor Protein, PI-Linked Cell-Surface.
Source:HEK293 Cells
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:GFRA1 filtered (0.4 �m) and lyophilized from 0.5mg/ml in phosphate buffered saline pH 7.5 containing 5 % (w/v) trehalose.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. GFRA1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGAS H HHHHHHHHH

GDNF family receptor alpha-1 (GFRA1) belongs to the GDNF receptor family. GFRA1 is a glycosyl-phosphatidylinositol(GPI)-linked cell surface receptor for both Glial cell line-derived growth factor (GDNF), neurturin (NTN), and mediates activation of the RET tyrosine kinase receptor. The GFRA1 protein is a potent survival factor for central and peripheral neurons, and is vital for the development of kidneys and the enteric nervous system.

GFRA1 Human Recombinant produced in HEK293 cells is a single,�glycosylated polypeptide chain (a.a 25-423) containing 409 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 46.0kDa (calculated).�

UniProt Protein Function:GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function:glial cell line-derived neurotrophic factor receptor activity; receptor binding

Biological Process: axon guidance; cell surface receptor linked signal transduction

NCBI Summary:Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:P56159
NCBI GenInfo Identifier:20141405
NCBI Gene ID:2674
NCBI Accession:P56159.2
UniProt Secondary Accession:P56159,O15507, O43912, A8KA21,
UniProt Related Accession:P56159
Molecular Weight:
NCBI Full Name:GDNF family receptor alpha-1
NCBI Synonym Full Names:GDNF family receptor alpha 1
NCBI Official Symbol:GFRA1��
NCBI Official Synonym Symbols:GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1��
NCBI Protein Information:GDNF family receptor alpha-1; RET ligand 1; GDNFR-alpha-1; GDNF receptor alpha-1; GPI-linked anchor protein; PI-linked cell-surface accessory protein; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; Glial cell line-derived neurotrophic factor receptor alpha
UniProt Protein Name:GDNF family receptor alpha-1
UniProt Synonym Protein Names:RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
Protein Family:GDNF family receptor
UniProt Gene Name:GFRA1��
UniProt Entry Name:GFRA1_HUMAN
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