Enzymes Recombinant Proteins

Human GDA Recombinant Protein (RPPB1684)

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SKU:
RPPB1684
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y2T3
Research Area:
Enzymes

Description

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Product Name:Human GDA Recombinant Protein
Product Code:RPPB1684
Size:10µg
Species:Human
Target:GDA
Synonyms:Guanine Deaminase, Guanine Aminohydrolase, GuanineAminase, P51-Nedasin, EC 3.5.4.3, GUANASE, GAH, Cytoplasmic PSD95 Interactor,KIAA1258, NEDASIN, CYPIN.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GDA protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 10% glycerol, 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
Biological Activity:Specific activity is > 2,000 pmol/min/ug, and isdefined as the amount of enzyme that convert guanine to xanthine per minute atpH 8.0 at 37C.

GDA is a member of the ATZ/TRZ family and is in charge for the hydrolytic deamination of guanine. GDA takes part in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for GDA.

GDA Human Recombinant produced in E. coli is a single polypeptide chain containing 477 amino acids (1-454) and having a molecular mass of 53kDa.GDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GDA: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Belongs to the ATZ/TRZ family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; EC 3.5.4.3; Hydrolase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: intracellular; cytosol

Molecular Function:guanine deaminase activity; zinc ion binding

Biological Process: nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; guanine catabolic process; purine base metabolic process

NCBI Summary:This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:Q9Y2T3
NCBI GenInfo Identifier:9910736
NCBI Gene ID:9615
NCBI Accession:Q9Y2T3.1
UniProt Secondary Accession:Q9Y2T3,Q5SZC7, Q9H335, Q9ULG2, B4DTY5,
UniProt Related Accession:Q9Y2T3
Molecular Weight:454
NCBI Full Name:Guanine deaminase
NCBI Synonym Full Names:guanine deaminase
NCBI Official Symbol:GDA��
NCBI Official Synonym Symbols:CYPIN; GUANASE; NEDASIN��
NCBI Protein Information:guanine deaminase; GAH; p51-nedasin; guanine aminase; guanine aminohydrolase; cytoplasmic PSD95 interactor
UniProt Protein Name:Guanine deaminase
UniProt Synonym Protein Names:Guanine aminohydrolase; GAH; p51-nedasin
UniProt Gene Name:GDA��
UniProt Entry Name:GUAD_HUMAN
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