Enzymes Recombinant Proteins
Human GCAT Recombinant Protein (RPPB1679)
- SKU:
- RPPB1679
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O75600
- Research Area:
- Enzymes
Description
| Product Name: | Human GCAT Recombinant Protein |
| Product Code: | RPPB1679 |
| Size: | 20µg |
| Species: | Human |
| Target: | GCAT |
| Synonyms: | 2-amino-3-ketobutyrate coenzyme A ligase mitochondrial, AKB ligase, EC 2.3.1.29, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GCAT protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP |
L-threonine to glycine degradation consists of a two-step biochemical pathway which involvs the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is initially converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. Glycine C-Acetyltransferase (GCAT) is the 2nd enzyme in this pathway, which subsequently catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The GCAT enzyme is regard as a class II pyridoxal-phosphate-dependent aminotransferase. GCAT is strongly expressed in the heart, brain, liver and pancreas. GCAT is also found in lung.
GCAT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 419 amino acids (22-419 a.a) and having a molecular mass of 45kDa.GCAT is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010] |
| UniProt Protein Details: | Protein type:Transferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.29; Mitochondrial Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: mitochondrial inner membrane; mitochondrion; nucleoplasm Biological Process: threonine catabolic process to pyruvate |
| NCBI Summary: | The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010] |
| UniProt Code: | O75600 |
| NCBI GenInfo Identifier: | 6685545 |
| NCBI Gene ID: | 23464 |
| NCBI Accession: | O75600.1 |
| UniProt Secondary Accession: | O75600,Q6ZWF1, Q96CA9, E2QC23, |
| UniProt Related Accession: | O75600 |
| Molecular Weight: | 47,974 Da |
| NCBI Full Name: | 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial |
| NCBI Synonym Full Names: | glycine C-acetyltransferase |
| NCBI Official Symbol: | GCAT�� |
| NCBI Official Synonym Symbols: | KBL�� |
| NCBI Protein Information: | 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial |
| UniProt Protein Name: | 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial |
| UniProt Synonym Protein Names: | Aminoacetone synthase; Glycine acetyltransferase |
| UniProt Gene Name: | GCAT�� |
| UniProt Entry Name: | KBL_HUMAN |
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