Enzymes Recombinant Proteins

Human GCAT Recombinant Protein (RPPB1679)

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RPPB1679

Description

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Product Name:Human GCAT Recombinant Protein
Product Code:RPPB1679
Size:20µg
Species:Human
Target:GCAT
Synonyms:2-amino-3-ketobutyrate coenzyme A ligase mitochondrial, AKB ligase, EC 2.3.1.29, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:GCAT protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP

L-threonine to glycine degradation consists of a two-step biochemical pathway which involvs the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is initially converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. Glycine C-Acetyltransferase (GCAT) is the 2nd enzyme in this pathway, which subsequently catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The GCAT enzyme is regard as a class II pyridoxal-phosphate-dependent aminotransferase. GCAT is strongly expressed in the heart, brain, liver and pancreas. GCAT is also found in lung.

GCAT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 419 amino acids (22-419 a.a) and having a molecular mass of 45kDa.GCAT is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
UniProt Protein Details:

Protein type:Transferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.29; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: mitochondrial inner membrane; mitochondrion; nucleoplasm

Biological Process: threonine catabolic process to pyruvate

NCBI Summary:The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
UniProt Code:O75600
NCBI GenInfo Identifier:6685545
NCBI Gene ID:23464
NCBI Accession:O75600.1
UniProt Secondary Accession:O75600,Q6ZWF1, Q96CA9, E2QC23,
UniProt Related Accession:O75600
Molecular Weight:47,974 Da
NCBI Full Name:2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
NCBI Synonym Full Names:glycine C-acetyltransferase
NCBI Official Symbol:GCAT��
NCBI Official Synonym Symbols:KBL��
NCBI Protein Information:2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Protein Name:2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Synonym Protein Names:Aminoacetone synthase; Glycine acetyltransferase
UniProt Gene Name:GCAT��
UniProt Entry Name:KBL_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O75600
Research Area:
Enzymes
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