Enzymes Recombinant Proteins

Human GATC Recombinant Protein (RPPB1675)

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SKU:
RPPB1675
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O43716
Research Area:
Enzymes

Description

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Product Name:Human GATC Recombinant Protein
Product Code:RPPB1675
Size:10µg
Species:Human
Target:GATC
Synonyms:Glutamyl-TRNA(Gln) Amidotransferase Subunit C, Glu-AdTSubunit C, 15E1.2, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Homolog(Bacterial), Glutamyl-TRNA(Gln) Amidotransferase Subunit C Mitochondrial, Glutamyl-TRNA(Gln)Amidotransferase Subunit C Homolog, EC 6.3.5, Protein 15E1.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:GATC protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVDFGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVAPPGNISLPKL DEQEPFPHS

Glutamyl-TRNAAmidotransferase, Subunit C also known as GATC allows the formation of properlycharged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) inthe mitochondria. The reaction occurs in the attendance of glutamine and ATP throughan activated gamma-phospho-Glu-tRNA(Gln). In addition, GATC is subunit of theheterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112)and C (GATC) subunits.

GATC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear higher).GATC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GATC: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatC family.
UniProt Protein Details:

Protein type:Mitochondrial; EC 6.3.5.-

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: mitochondrion

Molecular Function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; protein binding

Biological Process: mitochondrial translation

UniProt Code:O43716
NCBI GenInfo Identifier:76828060
NCBI Gene ID:283459
NCBI Accession:BC107145
UniProt Secondary Accession:O43716,Q3B824, Q3KNR8, B3KSU7,
UniProt Related Accession:O43716
Molecular Weight:15,086 Da
NCBI Full Name:Homo sapiens glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial), mRNA
NCBI Synonym Full Names:glutamyl-tRNA amidotransferase subunit C
NCBI Official Symbol:GATC��
NCBI Official Synonym Symbols:15E1.2��
NCBI Protein Information:glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial
UniProt Protein Name:Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial
UniProt Synonym Protein Names:Protein 15E1.2
Protein Family:PTS system galactitol-specific EIIC component
UniProt Gene Name:GATC��
UniProt Entry Name:GATC_HUMAN
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